ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 2, pp. 249–252. © Pleiades Publishing, Inc., 2010.
Original Russian Text © M.A. Sorokin, S.P. Medvedev, A.I. Shevchenko, N.M. Slynko, S.M. Zakian, 2010, published in Genetika, 2010, Vol. 46, No. 2, pp. 282–286.
Division and differentiation of cell lines in preim
plantation mammalian embryo at the morula and
blastocyst stages are a result of the interaction of pro
teins encoded by several key genes known as early
developmental genes . Of great interest among
them are genes encoding transcription factors respon
sible for the expression of many other genes and finally
determining specific properties of three cell types
comprising the blastocyst: epiblast, hypoblast and tro
phoectoderm . Three stem cell types can be
obtained from the mouse blastocyst: embryonic stem
(ES) cells, extraembryonic endoderm stem cells and
trophoblast stem (TS) cells. The properties of these
cell lines are also determined by the expression of par
ticular early developmental genes in them .
Previously, genes of vole
encoding transcription factors
demonstrating important functions in the system of
pluripotent state maintenance in epiblast and embry
onic stem cells (ESC) have been studied in detail
[2, 3]. The present study focuses on the expression of
other genes encoding vole transcription factors:
Klf4, Myc, Sall4, Gata6, Foxa2, Hnf4a, Cdx2, Esrrb,
. These genes function in other mammals not
only at the early developmental stages, but also at the
late stages of ontogeny; moreover, specific expression
patterns in the organs of adult animals was reported.
We have studied the expression pattern in the organs of
mature voles, previously obtained trophoblast stem
(TS) cells and extraembryonic endoderm (XEN) cells
[4, 5], aiming at detecting genes that could be further
used as markers to characterize other similar cell lines.
Vole and mouse cell lines (Table 1), vole embryos at
7 and 9 days post coitum and biopsy from the organs of
adult animals (large cerebral hemispheres, heart, lung,
stomach, liver, colon, kidney, ovary, and testicle)
served as the material for the expression research.
Embryos and biopsy materials were obtained from ani
mals of the laboratory population of
maintained in the vivarium of the Institute of
Cytology and Genetics, Russian Academy. RNA was
isolated using TRIREAGENT (MRC) from the cell
lines and organs. The extracted RNA was subjected to
reverse transcription with random primers.
Expression of Early Developmental Genes
M. A. Sorokin, S. P. Medvedev, A. I. Shevchenko,
N. M. Slynko, and S. M. Zakian
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090;
Received June 8, 2009.
—The expression of genes
lines, embryos and organs of adult voles
was studied. High resemblance of the
expression patterns of these genes in the organs of adult voles, mice and humans was demonstrated. It was
established that genes
were specifically expressed in vole extraembryonic endoderm
genes, in trophoblast stem cells. This shows that these genes can be used as mark
ers of corresponding vole cell lines. Indirect confirmation pointing to the fact that
gene is a marker gene
for epiblast cells both in the vole and mouse was obtained.
Cell lines used in the study
Cell type Line Karyotype Passage
Vole extraembryonic endoderm stem cells XEN M2 2
, XY 8
Vole trophoblast stem cells R1 2
, X0 13–15
Vole trophoblast stem cells R2 2
, XX 30
Vole embryonic fibroblasts –
Mouse embryonic fibroblasts –
Mouse embryonic stem cells MESBl6 2
, XX 31