Expression of cancer-testis antigens of Mage-a and Mage-b families in mouse embryonic fibroblasts cultured in vitro

Expression of cancer-testis antigens of Mage-a and Mage-b families in mouse embryonic fibroblasts... Cancer-testis antigens are expressed in the spermatogenic and cancer cells, as well as in human and mouse pluripotent stem cells. However, the role of cancer-testis antigens of Mage families in the regulation of cellular processes in embryonic cells is largely unknown. In the present study, a comparative quantitative analysis of the Mage-a and Mage-b gene expression was performed in mouse embryonic somatic cells (mouse embryonic fibroblasts, MEFs) long-term cultured in vitro or exposed to factors that inhibit and stimulate proliferation. The analysis revealed a low expression of cancer-testis antigens of Mage families and showed that the decrease in proliferative activity of MEFs at late passages was accompanied by slight up-regulation of the Mage-a gene expression and down-regulation of Mage-b gene expression. However, modulation of the MEK/ERK-signaling pathway activity and DNA demethylation with 5-azacytidine had no significant effects on the Mage-a and Mage-b gene expression in MEFs. The most essential changes in the expression levels of Mage-a and Mage-b genes were found only when MEFs were exposed to mitomycin C. In all experimental variants, the predominant cytoplasmic localization of Mage antigens was found in MEFs at the DNA synthesis stage, as well as at other stages of the cell cycle. Presumably, in actively proliferating mouse embryonic somatic cells, the antigens of Mage-a and Mage-b families can act as coactivators in the regulation of cell proliferation and other cellular processes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Expression of cancer-testis antigens of Mage-a and Mage-b families in mouse embryonic fibroblasts cultured in vitro

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360415030030
Publisher site
See Article on Publisher Site

Abstract

Cancer-testis antigens are expressed in the spermatogenic and cancer cells, as well as in human and mouse pluripotent stem cells. However, the role of cancer-testis antigens of Mage families in the regulation of cellular processes in embryonic cells is largely unknown. In the present study, a comparative quantitative analysis of the Mage-a and Mage-b gene expression was performed in mouse embryonic somatic cells (mouse embryonic fibroblasts, MEFs) long-term cultured in vitro or exposed to factors that inhibit and stimulate proliferation. The analysis revealed a low expression of cancer-testis antigens of Mage families and showed that the decrease in proliferative activity of MEFs at late passages was accompanied by slight up-regulation of the Mage-a gene expression and down-regulation of Mage-b gene expression. However, modulation of the MEK/ERK-signaling pathway activity and DNA demethylation with 5-azacytidine had no significant effects on the Mage-a and Mage-b gene expression in MEFs. The most essential changes in the expression levels of Mage-a and Mage-b genes were found only when MEFs were exposed to mitomycin C. In all experimental variants, the predominant cytoplasmic localization of Mage antigens was found in MEFs at the DNA synthesis stage, as well as at other stages of the cell cycle. Presumably, in actively proliferating mouse embryonic somatic cells, the antigens of Mage-a and Mage-b families can act as coactivators in the regulation of cell proliferation and other cellular processes.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: May 26, 2015

References

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