To better understand the process of fluid movement driven by Cl− conductance, a Cl− channel-forming peptide was delivered to the luminal membrane of microperfused rabbit renal proximal tubules. When the peptide (NK4-M2GlyR) was perfused, a significant new conductance was observed within 3 min and stabilized at 10 min. Alteration of the ion composition revealed it to be a Cl−-specific conductance. Reabsorption of Cl− (J Cl) was increased by NK4-M2GlyR, but not by a scramble NK4-M2GlyR sequence, suggesting that the active peptide formed de novo Cl− channels in the luminal membrane of the perfused tubules. In the presence of the peptide, reabsorption of fluid (J v) was dramatically increased and J Na and J Ca were concomitantly increased. We propose that introduction of the new Cl− conductance in the luminal membrane leads to a coordinated efflux of water across the membrane and an increase in cation translocation via the paracellular pathway, resulting in an increase in J v. This novel method could prove useful in characterizing mechanisms of fluid transport driven by Cl− gradients.
The Journal of Membrane Biology – Springer Journals
Published: Jun 1, 2003
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