Expression of acylamidase gene in Rhodococcus erythropolis strains

Expression of acylamidase gene in Rhodococcus erythropolis strains The expression of a new acylamidase gene from R. erythropolis TA37 was studied in Rhodococcus erythropolis strains. This acylamidase, as a result of its unique substrate specificity, can hydrolyse N-substituted amides (4′-nitroacetanilide, N-isopropylacrylamide, N′N-dimethylaminopropylacrylamide). A new expression system based on the use of the promoter region of nitrile hydratase genes from R. rhodochrous M8 was created to achieve constitutive synthesis of acylamidase in R. erythropolis cells. A fourfold improvement in the acylamidase activity of recombinant R. erythropolis cells as compared with the parent wild-type strain was obtained through the use of the new expression system. Russian Journal of Genetics Springer Journals

Expression of acylamidase gene in Rhodococcus erythropolis strains

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Pleiades Publishing
Copyright © 2014 by Pleiades Publishing, Inc.
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
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