Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site

Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site The promoter of a gene encoding a ribosome-associated protein of 40 kDa from Arabidopsis thaliana (A-p40) was sequenced and the expression of the gene studied. A-p40 was expressed in the same organs and with the same variations as the eukaryotic elongation factor 1α (eEF1A), another gene coding for a protein involved in translation Arabidopsis plants transformed with a β-glucuronidase (GUS) gene driven by the A-p40 promoter confirm that A-p40 is expressed in actively dividing and growing cells. eEF1A promoter-GUS fusions have the same pattern of expression. Comparison of cis-acting elements from A-p40 and eEF1A revealed some common elements. A-p40 promoter deletions and transient gene expression in transfected Arabidopsis protopasts allowed the identification of trap40, a cis-acting element regulating gene expression. Gel retardation experiments indicate that eEF1A and A-p40 are regulated by different cis-acting elements. The role of such elements is discussed. Plant Molecular Biology Springer Journals

Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site

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Kluwer Academic Publishers
Copyright © 1997 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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