Expression kinetics of the late UL12 gene encoding the bovine herpesvirus 1 alkaline nuclease

Expression kinetics of the late UL12 gene encoding the bovine herpesvirus 1 alkaline nuclease We characterized the expression kinetics of the transcript and protein generated from the bovine herpesvirus 1 (BHV1) homologue of the herpes simplex virus 1 (HSV1) UL12 gene that encodes a viral alkaline nuclease. The BHV1 UL12 coding sequence, which was previously shown to express in E. coli a protein exhibiting nuclease activity, is located at positions 82776 → 84239 of the viral genome. Northern blot analysis of RNA from BHV1-infected cells with a single strand RNA probe complementary to UL12 detected four specific 3′ coterminal viral transcripts of 4.2, 3.7, 2.2, and 0.7 kb that accumulated simultaneously from 6 to 24 hours post-infection (p.i.). S1 nuclease mapping of the UL12 capping site at position 82384 of the genome as well as the identification of a consensus polyadenylation signal at 84490–84495 allowed us to establish that the 2.2 kb transcript corresponds to that of UL12. A UL12 specific antiserum generated against a T7-Tag/UL12 fusion protein expressed in E. coli detected a 53 kDa protein in cell lysates from BHV1-infected cells, whose size correlated with that predicted (51,844 Da), which accumulated from 12 to 30 h p.i. Differences observed between the transcriptional and translational expression profiles suggest that the UL12 of BHV1 is regulated at both the translational and post-translational levels. Surprisingly, the protein expression was strictly dependent on viral DNA synthesis, unambiguously demonstrating that BHV1 UL12 belongs to viral genes of the γ2 class. This is in contrast to the HSV1 and pseudorabies homologues that are classified as early (β) genes. Further studies will be required to determine whether these kinetic differences have any functional implications. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Expression kinetics of the late UL12 gene encoding the bovine herpesvirus 1 alkaline nuclease

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Publisher
Springer-Verlag
Copyright
Copyright © 2001 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050170039
Publisher site
See Article on Publisher Site

Abstract

We characterized the expression kinetics of the transcript and protein generated from the bovine herpesvirus 1 (BHV1) homologue of the herpes simplex virus 1 (HSV1) UL12 gene that encodes a viral alkaline nuclease. The BHV1 UL12 coding sequence, which was previously shown to express in E. coli a protein exhibiting nuclease activity, is located at positions 82776 → 84239 of the viral genome. Northern blot analysis of RNA from BHV1-infected cells with a single strand RNA probe complementary to UL12 detected four specific 3′ coterminal viral transcripts of 4.2, 3.7, 2.2, and 0.7 kb that accumulated simultaneously from 6 to 24 hours post-infection (p.i.). S1 nuclease mapping of the UL12 capping site at position 82384 of the genome as well as the identification of a consensus polyadenylation signal at 84490–84495 allowed us to establish that the 2.2 kb transcript corresponds to that of UL12. A UL12 specific antiserum generated against a T7-Tag/UL12 fusion protein expressed in E. coli detected a 53 kDa protein in cell lysates from BHV1-infected cells, whose size correlated with that predicted (51,844 Da), which accumulated from 12 to 30 h p.i. Differences observed between the transcriptional and translational expression profiles suggest that the UL12 of BHV1 is regulated at both the translational and post-translational levels. Surprisingly, the protein expression was strictly dependent on viral DNA synthesis, unambiguously demonstrating that BHV1 UL12 belongs to viral genes of the γ2 class. This is in contrast to the HSV1 and pseudorabies homologues that are classified as early (β) genes. Further studies will be required to determine whether these kinetic differences have any functional implications.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2001

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