The sites of desaturation in plant cells were studied by expressing the fusion gene composed of the genes encoding FAD2 (Δ12 desaturase) from spinach (Spinacia oleracea) and enhanced green fluorescent protein (EGFP) under the control of the 35S cauliflower mosaic virus promoter. The chimeric protein was functional in tobacco (Nicotiana tabacum) BY-2 cells. The temporal changes in distribution and localization of fusion FAD2-EGFP protein were studied. According to the results obtained, we can conclude that the sites of desaturation in plant cells are associated with both the endoplasmic reticulum and plasma membrane.
Russian Journal of Plant Physiology – Springer Journals
Published: Jul 12, 2008
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