Expression and Functional Characterization of the Human Ether-à-go-go-Related Gene (HERG) K+ Channel Cardiac Splice Variant in Xenopus laevis Oocytes

Expression and Functional Characterization of the Human Ether-à-go-go-Related Gene (HERG) K+... HERG CCardiac, a C-terminal splice variant of the human ether-à-go-go-related gene (HERG A), was identified and found to be 100% homologous to HERGUSO. Real-time polymerase chain reaction data indicated that in the human heart HERG CCardiac mRNA was expressed eight times more than HERG A, whereas in human ventricular tissue it was expressed six times more than HERG A. A HERG CCardiac-green fluorescence protein (GFP) construct was heterologously expressed in Xenopus oocytes. Confocal micrographs revealed that HERG CCardiac was mainly expressed in the plasma membrane. HERG CCardiac channel expressed in oocytes produced slower inactivating outward currents and faster deactivating tail currents than those of HERG A channel. Equal amounts of HERG A and HERG CCardiac cRNA coinjected into oocytes formed intermediate HERG A + HERG CCardiac heteromultimers, which was reconfirmed by immunoprecipitation experiments with a HERG A N-terminal antibody. These heteromultimers had different inactivation, deactivation and activation kinetics from those of HERG A and HERG CCardiac channels. HERG A + HERG CCardiac heteromultimers significantly reduced the model action potential mean amplitude and increased the fast and slow inactivation τ values of the action potential repolarization phase, suggesting involvement of HERG A and HERG CCardiac heteromultimers in modulation of the refractory interval. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Expression and Functional Characterization of the Human Ether-à-go-go-Related Gene (HERG) K+ Channel Cardiac Splice Variant in Xenopus laevis Oocytes

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Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-006-0010-9
Publisher site
See Article on Publisher Site

Abstract

HERG CCardiac, a C-terminal splice variant of the human ether-à-go-go-related gene (HERG A), was identified and found to be 100% homologous to HERGUSO. Real-time polymerase chain reaction data indicated that in the human heart HERG CCardiac mRNA was expressed eight times more than HERG A, whereas in human ventricular tissue it was expressed six times more than HERG A. A HERG CCardiac-green fluorescence protein (GFP) construct was heterologously expressed in Xenopus oocytes. Confocal micrographs revealed that HERG CCardiac was mainly expressed in the plasma membrane. HERG CCardiac channel expressed in oocytes produced slower inactivating outward currents and faster deactivating tail currents than those of HERG A channel. Equal amounts of HERG A and HERG CCardiac cRNA coinjected into oocytes formed intermediate HERG A + HERG CCardiac heteromultimers, which was reconfirmed by immunoprecipitation experiments with a HERG A N-terminal antibody. These heteromultimers had different inactivation, deactivation and activation kinetics from those of HERG A and HERG CCardiac channels. HERG A + HERG CCardiac heteromultimers significantly reduced the model action potential mean amplitude and increased the fast and slow inactivation τ values of the action potential repolarization phase, suggesting involvement of HERG A and HERG CCardiac heteromultimers in modulation of the refractory interval.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Oct 14, 2006

References

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