Exploring expression patterns of PR-1, PR-2, PR-3, and PR-12 like genes in Arabidopsis thaliana upon Alternaria brassicae inoculation

Exploring expression patterns of PR-1, PR-2, PR-3, and PR-12 like genes in Arabidopsis thaliana... In this study, we systematically examined the expression patterns of pathogenesis-related genes in model plant Arabidopsis thaliana after Alternaria brassicae inoculation using reverse transcription polymerase chain reaction(RT-PCR). Based on the results, none of the PR-1 and PR-2 like genes were induced significantly in the unwounded local or distal leaves upon A. brassicae challenge. However, only At2g14580 of the PR-1like gene showed a significant expression in wounded leave after Alternaria challenge but not in control; confirming its expression in response to A. brassicae was aided by the wounding. Among PR-3 like genes, At2g43590 showed local early expression and other PR-3 like genes showed significant distal expression after A. brassicae infection only in unwounded but not in wounded leaf samples. Although all the three PR-12 like genes were induced in local tissues, At2g26020 was the only gene showed significant induction locally as well as systemically after pathogen infection in the both with and without wounding experiments. Therefore, among the PR-1, PR-2, PR-3 and PR-12 like genes studied, At2g26020 can be the most promising candidate for the further line of research, viz, molecular characterization of its promoter to develop pathogen-inducible promoter in response to Alternaria and to develop fungus-resistant transgenics in Brassica juncea. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png 3 Biotech Springer Journals

Exploring expression patterns of PR-1, PR-2, PR-3, and PR-12 like genes in Arabidopsis thaliana upon Alternaria brassicae inoculation

Loading next page...
 
/lp/springer_journal/exploring-expression-patterns-of-pr-1-pr-2-pr-3-and-pr-12-like-genes-cj89dsRFDf
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials
ISSN
2190-572X
eISSN
2190-5738
D.O.I.
10.1007/s13205-018-1259-2
Publisher site
See Article on Publisher Site

Abstract

In this study, we systematically examined the expression patterns of pathogenesis-related genes in model plant Arabidopsis thaliana after Alternaria brassicae inoculation using reverse transcription polymerase chain reaction(RT-PCR). Based on the results, none of the PR-1 and PR-2 like genes were induced significantly in the unwounded local or distal leaves upon A. brassicae challenge. However, only At2g14580 of the PR-1like gene showed a significant expression in wounded leave after Alternaria challenge but not in control; confirming its expression in response to A. brassicae was aided by the wounding. Among PR-3 like genes, At2g43590 showed local early expression and other PR-3 like genes showed significant distal expression after A. brassicae infection only in unwounded but not in wounded leaf samples. Although all the three PR-12 like genes were induced in local tissues, At2g26020 was the only gene showed significant induction locally as well as systemically after pathogen infection in the both with and without wounding experiments. Therefore, among the PR-1, PR-2, PR-3 and PR-12 like genes studied, At2g26020 can be the most promising candidate for the further line of research, viz, molecular characterization of its promoter to develop pathogen-inducible promoter in response to Alternaria and to develop fungus-resistant transgenics in Brassica juncea.

Journal

3 BiotechSpringer Journals

Published: Apr 27, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off