Experimental infection of king crab hemocytes with a herpes-like virus in culture

Experimental infection of king crab hemocytes with a herpes-like virus in culture To date, herpes-like viruses have been repeatedly found in wild king crab populations. In this study, freshly collected hemocytes of the red king crab Paralithodes camtschaticus have been experimentally exposed in culture to the herpes-like virus from thawed antennal gland samples from infected blue king crabs P. platypus. After 3–4 months of cultivation, significant differences in the pigmentation were observed between control and virus-infected hemocytes. Using Raman spectroscopy, we detected two obligatory peaks, characteristic for melanin identification, in the spectra of pigmented inclusions in harvested virus-infected hemocytes. These peaks are absent in the spectra obtained from control hemocytes. The cells of virus-infected cultures were viable after 10 months, while all control uninfected hemocytes were lost. As demonstrated by electron microscopic studies, virus-like particles become abundant at 7 days post-inoculation. Our results suggest that hemocytes could be used as a suitable model system for studies of crab pathogens. The in vitro studies confirm previous findings regarding the presence of viral infection in the natural population of king crabs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Experimental infection of king crab hemocytes with a herpes-like virus in culture

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074015050107
Publisher site
See Article on Publisher Site

Abstract

To date, herpes-like viruses have been repeatedly found in wild king crab populations. In this study, freshly collected hemocytes of the red king crab Paralithodes camtschaticus have been experimentally exposed in culture to the herpes-like virus from thawed antennal gland samples from infected blue king crabs P. platypus. After 3–4 months of cultivation, significant differences in the pigmentation were observed between control and virus-infected hemocytes. Using Raman spectroscopy, we detected two obligatory peaks, characteristic for melanin identification, in the spectra of pigmented inclusions in harvested virus-infected hemocytes. These peaks are absent in the spectra obtained from control hemocytes. The cells of virus-infected cultures were viable after 10 months, while all control uninfected hemocytes were lost. As demonstrated by electron microscopic studies, virus-like particles become abundant at 7 days post-inoculation. Our results suggest that hemocytes could be used as a suitable model system for studies of crab pathogens. The in vitro studies confirm previous findings regarding the presence of viral infection in the natural population of king crabs.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: Nov 4, 2015

References

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