Expansion of the pig comparative map by expressed sequence tags (EST) mapping

Expansion of the pig comparative map by expressed sequence tags (EST) mapping We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-stranded conformational polymorphisms developed in 3′ untranslated regions of transcripts. For 20 of the transcripts, the human homolog has already been mapped. This study thus represents a significant contribution to the pig comparative map. Some important findings were that we could clarify the extent of a previously identified inversion event in a region of conserved synteny between SSC6q and HSAlp, that SSC14q does contain a region homologous to HS Al, a situation not clear from earlier ZOO-FISH studies, and that the homology between SSCI7 and HSA20 includes the p-arm of HSA20. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Expansion of the pig comparative map by expressed sequence tags (EST) mapping

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Publisher
Springer-Verlag
Copyright
Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900609
Publisher site
See Article on Publisher Site

Abstract

We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-stranded conformational polymorphisms developed in 3′ untranslated regions of transcripts. For 20 of the transcripts, the human homolog has already been mapped. This study thus represents a significant contribution to the pig comparative map. Some important findings were that we could clarify the extent of a previously identified inversion event in a region of conserved synteny between SSC6q and HSAlp, that SSC14q does contain a region homologous to HS Al, a situation not clear from earlier ZOO-FISH studies, and that the homology between SSCI7 and HSA20 includes the p-arm of HSA20.

Journal

Mammalian GenomeSpringer Journals

Published: Mar 21, 2009

References

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