Mammalian Genome 8, 785–786 (1997). © Springer-Verlag New York Inc. 1997 Exclusion of Ifa and Ifb as the Lps gene and mapping of three markers near the Lps locus 1 2 2 1 1 Stacia Peiffer-Schneider, Brian C. Schutte, Jeffrey C. Murray, Kathy L. Frees, Kimberly Williamson, 2 1 Nancy J. Leysens, David A. Schwartz Department of Internal Medicine, Pulmonary, Critical Care and Occupational Medicine Division, College of Medicine, The University of Iowa, Iowa City, IA 52242, USA Department of Pediatrics, The University of Iowa, College of Medicine, Iowa City, Iowa 52242, USA Received: 15 April 1997 / Accepted: 5 June 1997 The lipopolysaccharide (Lps) locus in mice controls the biological http://www.ncbi.nlm.nih.gov/) were developed to specifically am- response to endotoxin, the lipopolysaccharide (LPS) from the cell plify 300 bp overlapping segments of each of the eleven known Ifa wall of Gram-negative bacteria. The Lps locus has been shown to and Ifb genes. Each gene segment was amplified from DNA iso- be a single Mendelian gene that maps to Chromosome (Chr) 4 lated from C3H/HeJ mice and LPS-sensitive C3H/OuJ mice by the (Watson et al. 1978). A LPS hyporesponsive mouse arose spon- polymerase chain reaction (PCR). These strains are closely
Mammalian Genome – Springer Journals
Published: Oct 1, 1997
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