Evaluation of indicators of postejaculation maturation of spermatozoa of Bos taurus using a chlortetracycline test

Evaluation of indicators of postejaculation maturation of spermatozoa of Bos taurus using a... Postejaculation maturation of spermatozoa (capacitation and acrosomal exocytosis) in bovine sperm was assessed using the method of staining with chlortetracycline and inhibitor analysis to identify the transition ways of calcium of intracellular depots under the influence of highly dispersed silica. It was shown that highly dispersed silica in a concentration of 0.001% stimulates capacitation but has no impact on the acrosome exocytosis in bovine sperm. Activated by highly dispersed silica, capacitation was inhibited in the presence of cytochalasin D and H-89 inhibitors, whereas nocodazole and Ro 31-8220 had no influence on this process. The joint action of theophylline and guanosine diphosphate stimulates an increase in the amount of capacitated sperm cells, similarly to highly dispersed silica; inhibitors cytochalasin D and H-89 restrict the capacitation of sperm activated by these compounds. At the same time, the joint effect of prolactin and guanosine triphosphate had no effect on the capacitation of spermatozoa; addition of nocodazole and Ro 31-8220 inhibitors did not alter the effect of prolactin and guanosine triphosphate on the capacitation of sperm. A hypothesis was put forward, according to which increase in the cryoresistance of spermatozoa of bulls under the influence of highly dispersed silica is, apparently, determined by the transition of Ca2+ between the intracellular stores in the direction from inositol triphosphate-sensitive to inositol triphosphate-insensitive intracellular stores of calcium. The obtained data allow us to expand the notion of the biochemical mechanisms of capacitation in the spermatozoa of bulls. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Evaluation of indicators of postejaculation maturation of spermatozoa of Bos taurus using a chlortetracycline test

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360415060028
Publisher site
See Article on Publisher Site

Abstract

Postejaculation maturation of spermatozoa (capacitation and acrosomal exocytosis) in bovine sperm was assessed using the method of staining with chlortetracycline and inhibitor analysis to identify the transition ways of calcium of intracellular depots under the influence of highly dispersed silica. It was shown that highly dispersed silica in a concentration of 0.001% stimulates capacitation but has no impact on the acrosome exocytosis in bovine sperm. Activated by highly dispersed silica, capacitation was inhibited in the presence of cytochalasin D and H-89 inhibitors, whereas nocodazole and Ro 31-8220 had no influence on this process. The joint action of theophylline and guanosine diphosphate stimulates an increase in the amount of capacitated sperm cells, similarly to highly dispersed silica; inhibitors cytochalasin D and H-89 restrict the capacitation of sperm activated by these compounds. At the same time, the joint effect of prolactin and guanosine triphosphate had no effect on the capacitation of spermatozoa; addition of nocodazole and Ro 31-8220 inhibitors did not alter the effect of prolactin and guanosine triphosphate on the capacitation of sperm. A hypothesis was put forward, according to which increase in the cryoresistance of spermatozoa of bulls under the influence of highly dispersed silica is, apparently, determined by the transition of Ca2+ between the intracellular stores in the direction from inositol triphosphate-sensitive to inositol triphosphate-insensitive intracellular stores of calcium. The obtained data allow us to expand the notion of the biochemical mechanisms of capacitation in the spermatozoa of bulls.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Nov 18, 2015

References

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