Evaluation of Genetic Relatedness Among Temperate Pome Fruit Crops of Family Rosaceae Using Arbitrary Oligonucleotide Markers

Evaluation of Genetic Relatedness Among Temperate Pome Fruit Crops of Family Rosaceae Using... A rationale use of the genetic resources in breeding programs requires an understanding of relationships within and among related crop varieties. In the present study, genetic relatedness among fifty genotypes of temperate pome fruit crops (apple, pear, quince and loquat) of family Rosaceae growing in Himachal Pradesh (India) was tested using arbitrary oligonucleotide random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular marker analyses. The level of polymorphism across the subjected genotypes was 97.29 and 97.45 % by RAPD and ISSR markers, respectively. The dendrograms derived from RAPD and ISSR analysis grouped temperate pome fruit crops into two major clusters comprising of 40 and 10 genotypes which branched at similarity value of 0.31 and 0.33, respectively. Estimates of pooled similarity coefficient ranging from 0.30 to 0.94 indicated their promise for application to future molecular screening, map construction and comparative genomic studies, etc. Therefore, these results could be helpful in framing more extensive studies on the determined taxa. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Evaluation of Genetic Relatedness Among Temperate Pome Fruit Crops of Family Rosaceae Using Arbitrary Oligonucleotide Markers

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Publisher
Springer Journals
Copyright
Copyright © 2016 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
D.O.I.
10.1007/s40011-016-0746-7
Publisher site
See Article on Publisher Site

Abstract

A rationale use of the genetic resources in breeding programs requires an understanding of relationships within and among related crop varieties. In the present study, genetic relatedness among fifty genotypes of temperate pome fruit crops (apple, pear, quince and loquat) of family Rosaceae growing in Himachal Pradesh (India) was tested using arbitrary oligonucleotide random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular marker analyses. The level of polymorphism across the subjected genotypes was 97.29 and 97.45 % by RAPD and ISSR markers, respectively. The dendrograms derived from RAPD and ISSR analysis grouped temperate pome fruit crops into two major clusters comprising of 40 and 10 genotypes which branched at similarity value of 0.31 and 0.33, respectively. Estimates of pooled similarity coefficient ranging from 0.30 to 0.94 indicated their promise for application to future molecular screening, map construction and comparative genomic studies, etc. Therefore, these results could be helpful in framing more extensive studies on the determined taxa.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: Jun 2, 2016

References

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