Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D architecture and surface chemistry

Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D... in order to explain the bioactivity of different protein for- mulations. We have shown that alteration of the chemical identity, conformation and availability of free binding motifs (GxOGER and RGD), resulting from addition of gelatin to collagen and crosslinking, have a profound effect on the ability of cells to adhere to these formulations. Carbodiimide crosslinking ablates integrin-dependent cell activity on both two-dimensional and three-dimensional architectures while the three-dimensional scaffold structure also leads to a high level of non-specific interactions remaining on three-dimensional samples even after a 1 Introduction The original version of this article was revised: the copyright was wrong in the PDF version of the article and the Open Access license The extracellular matrix (ECM) of tissues provides terms were missing. mechanical support for cells and supplies correct biological * Natalia Davidenko signals for cell activity [1–4]. When used as cell-delivery nd313@cam.ac.uk vehicles in tissue engineering (TE) applications, biopolymer scaffolds should mimic these ECM functions. Biological Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, UK performance of three-dimensional (3D) matrices are influ- enced by several parameters such as the nature and avail- Department of Biochemistry, University of Cambridge, Downing Site, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Materials Science: Materials in Medicine Springer Journals

Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D architecture and surface chemistry

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Publisher
Springer Journals
Copyright
Copyright © 2016 by The Author(s)
Subject
Materials Science; Biomaterials; Biomedical Engineering; Regenerative Medicine/Tissue Engineering; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Surfaces and Interfaces, Thin Films
ISSN
0957-4530
eISSN
1573-4838
D.O.I.
10.1007/s10856-016-5763-9
Publisher site
See Article on Publisher Site

Abstract

in order to explain the bioactivity of different protein for- mulations. We have shown that alteration of the chemical identity, conformation and availability of free binding motifs (GxOGER and RGD), resulting from addition of gelatin to collagen and crosslinking, have a profound effect on the ability of cells to adhere to these formulations. Carbodiimide crosslinking ablates integrin-dependent cell activity on both two-dimensional and three-dimensional architectures while the three-dimensional scaffold structure also leads to a high level of non-specific interactions remaining on three-dimensional samples even after a 1 Introduction The original version of this article was revised: the copyright was wrong in the PDF version of the article and the Open Access license The extracellular matrix (ECM) of tissues provides terms were missing. mechanical support for cells and supplies correct biological * Natalia Davidenko signals for cell activity [1–4]. When used as cell-delivery nd313@cam.ac.uk vehicles in tissue engineering (TE) applications, biopolymer scaffolds should mimic these ECM functions. Biological Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, UK performance of three-dimensional (3D) matrices are influ- enced by several parameters such as the nature and avail- Department of Biochemistry, University of Cambridge, Downing Site,

Journal

Journal of Materials Science: Materials in MedicineSpringer Journals

Published: Aug 31, 2016

References

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