Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation

Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of... Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency. Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-016-2903-y
Publisher site
See Article on Publisher Site

Abstract

Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency. Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2.

Journal

Archives of VirologySpringer Journals

Published: May 28, 2016

References

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