Cell Mol Neurobiol (2018) 38:577 https://doi.org/10.1007/s10571-017-0495-z ERRATUM Erratum to: O-GlcNAc Glycosylation of nNOS Promotes Neuronal Apoptosis Following Glutamate Excitotoxicity 1 2 3 4 4 • • • • • Rongrong Chen Peipei Gong Tao Tao Yilu Gao Jianhong Shen 4 5 1 6 • • • Yaohua Yan Chengwei Duan Jun Wang Xiaojuan Liu Published online: 3 May 2017 Springer Science+Business Media New York 2017 Erratum to: Cell Mol Neurobiol compared with the sham group (a–g). In addition, nNOS DOI 10.1007/s10571-017-0477-1 directly interacts with OGT in brain tissue as indicated by reciprocal immunoprecipitation studies (h, i). Subse- The original version of this article unfortunately contained quently, immunoprecipitation studies showed that in PC12 the errors in the caption of Figures 3 and 4. The corrected cells treated with or without glutamate, nNOS interacted ﬁgure captions are given below: with OGT directly, and the interaction between nNOS and Fig. 3 The interaction between nNOS and OGT was OGT was enhanced by glutamate stimulation as well as recently reported (Ryu et al. 2016). As OGT catalyzes the O-GlcNAcylation of nNOS (j). Thus, we have sufﬁcient OGlcNAc modiﬁcation of nNOS during excitotoxicity, we reason to conclude that excitotoxicity induces the increase speculated whether nNOS interacts with OGT. in nNOS O-GlcNAcylation due to an enhanced interaction Immunoﬂuorescent staining showed that nNOS was between nNOS and OGT strongly co-localized with OGT 3 h after ischemia Fig. 4 Identiﬁcation of O-GlcNAc sites of nNOS. PC12 cells were transfected with WT or mutated nNOS and OGT for 36 h and then subjected to immunoprecipitation with The online version of the original article can be found under anti-Myc-Tag(19C2) and IgG antibody and then analyzed doi: 10.1007/s10571-017-0477-1 by western blot as well as the total lysate of PC12 cells (a) n =3 & Jun Wang email@example.com & Xiaojuan Liu firstname.lastname@example.org Department of Geriatric Medicine, Afﬁliated Hospital of Nantong University, Nantong 226001, Jiangsu, China Department of Neurosurgery, Comprehensive Surgical Laboratory, Afﬁliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, China Jiangsu Province Key Laboratory for Inﬂammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu, China Department of Neurosurgery, Afﬁliated Hospital of Nantong University, Nantong 226001, Jiangsu, China Department of Science and Education, Second People’s Hospital of Nantong, Nantong University, Nantong 226001, Jiangsu, China Department of Pathogen Biology, Nantong University, Nantong 226001, Jiangsu, China
Cellular and Molecular Neurobiology – Springer Journals
Published: May 3, 2017
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