J. Membrane Biol. 178, 71 (2000) The Journal of DOI: 10.1007/s002320010023 Membrane Biology © Springer-Verlag New York Inc. 2000 Erratum T. Gutsmann, M. Fix, J.W. Larrick, A. Wiese. Mechanisms of Action of Rabbit CAP18 on Monolayers and Liposomes Made from Endotoxins of Phospholipids. The Journal of Membrane Biology. 176:3 223–236 (2000) (DOI: 10.1007/s002320001092) Erroneously, for the calculation of concentrations and molecular areas the molecular mass of the whole CAP18 molecule (18 kDA) instead of its antibacterial active C terminus (3.8 kDa) has been used. Consequently, the given molar concentrations of CAP18 have to be multiplied by a factor of 4.7 and the molecular areas and the LPS:CAP18 binding stoichiometries have to be divided by the same factor. The revised Table 2 appears below. Table 2. Size and binding stoichiometry of intercalated rCAP18 molecules into F515 LPS or R45 LPS monolayers LPS/Subphase Area per molecule/nm [LPS]:[CAP18] (M/M) F515 LPS/SUB.: +0 mM MgCl (0.8 ± 0.1) (0.7 ± 0.1):1 F515 LPS/SUB.: +5 mM MgCl (0.8 ± 0.1) (1.1 ± 0.1):1 R45 LPS/SUB.: +0 mM MgCl (0.8 ± 0.1) (1.1 ± 0.1):1 R45 LPS/SUB.: +5 mM MgCl (0.95 ± 0.1) (1.3 ± 0.1):1 The monolayers were prepared on a subphase containing 100 mM KCl, 5 mM HEPES with varying concentrations of MgCl (first column) at pH 7 and a temperature of 37°C. The intercalation was −1 determined at a lateral pressure of 30 mN ? m .
The Journal of Membrane Biology – Springer Journals
Published: Mar 18, 2014
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