Equivalent Isopropanol Concentrations of Aromatic Amino Acids Interactions with Lipid Vesicles

Equivalent Isopropanol Concentrations of Aromatic Amino Acids Interactions with Lipid Vesicles We show that the interaction of aromatic amino acids with lipid bilayers can be characterized by conventional 1D $$^1$$ 1 H NMR spectroscopy using reference spectra obtained in isopropanol-d8/D $$_{2}$$ 2 O solutions. We demonstrate the utility of this method with three different peptides containing tyrosine, tryptophan, or phenylalanine amino acids in the presence of 1,2-dioleoyl-sn-glycero-3-phosphocholine or 1,2-dioleoyl-sn-glycero-3-phosphoserine lipid membranes. In each case, we determine an equivalent isopropanol concentration (EIC) for each hydrogen site of aromatic groups, in essence constructing a map of the chemical environment. These EIC maps provide information on relative affinities of aromatic side chains for either PC or PS bilayers and also inform on amino acid orientation preference when bound to membranes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Equivalent Isopropanol Concentrations of Aromatic Amino Acids Interactions with Lipid Vesicles

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Publisher
Springer US
Copyright
Copyright © 2015 by Springer Science+Business Media New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-015-9781-1
Publisher site
See Article on Publisher Site

Abstract

We show that the interaction of aromatic amino acids with lipid bilayers can be characterized by conventional 1D $$^1$$ 1 H NMR spectroscopy using reference spectra obtained in isopropanol-d8/D $$_{2}$$ 2 O solutions. We demonstrate the utility of this method with three different peptides containing tyrosine, tryptophan, or phenylalanine amino acids in the presence of 1,2-dioleoyl-sn-glycero-3-phosphocholine or 1,2-dioleoyl-sn-glycero-3-phosphoserine lipid membranes. In each case, we determine an equivalent isopropanol concentration (EIC) for each hydrogen site of aromatic groups, in essence constructing a map of the chemical environment. These EIC maps provide information on relative affinities of aromatic side chains for either PC or PS bilayers and also inform on amino acid orientation preference when bound to membranes.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Feb 19, 2015

References

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