EPR study of the effect, induced by zidovudine (AZT), on the membrane lipid dynamics in leukemic cell

EPR study of the effect, induced by zidovudine (AZT), on the membrane lipid dynamics in leukemic... Zidovudine (AZT) was the first drug approved by the FDA for treatment of HIV infections. To investigate the AZT effects on the physical properties of K562 cell membranes, experiments were performed by measuring an order parameter value in these cells, previously labelled with 16-DSA (16-doxyl-stearic acid) or 5-DSA (5-doxyl-stearic acid). The EPR spectra of the labelled fatty acid were used to detect the alteration in their freedom of motion and to provide indications of membrane fluidity. Two different parameters were calculated from EPR spectra for the 16-DSA and 5-DSA. Preliminary data indicate that, for both the probes used, these parameters were not significantly different in the control cells with respect to the AZT-treated ones. Control measurements, performed to test the sensitivity of the technique using the DMSO agent, showed a significant increase in the K562 membrane fluidity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

EPR study of the effect, induced by zidovudine (AZT), on the membrane lipid dynamics in leukemic cell

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Publisher
Brill Academic Publishers
Copyright
Copyright © 2002 by VSP 2002
Subject
Chemistry; Inorganic Chemistry; Physical Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1163/156856702320267145
Publisher site
See Article on Publisher Site

Abstract

Zidovudine (AZT) was the first drug approved by the FDA for treatment of HIV infections. To investigate the AZT effects on the physical properties of K562 cell membranes, experiments were performed by measuring an order parameter value in these cells, previously labelled with 16-DSA (16-doxyl-stearic acid) or 5-DSA (5-doxyl-stearic acid). The EPR spectra of the labelled fatty acid were used to detect the alteration in their freedom of motion and to provide indications of membrane fluidity. Two different parameters were calculated from EPR spectra for the 16-DSA and 5-DSA. Preliminary data indicate that, for both the probes used, these parameters were not significantly different in the control cells with respect to the AZT-treated ones. Control measurements, performed to test the sensitivity of the technique using the DMSO agent, showed a significant increase in the K562 membrane fluidity.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Oct 13, 2004

References

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