Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China

Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus . The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2255-4
Publisher site
See Article on Publisher Site

Abstract

Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus . The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2015

References

  • Circovirus infections in pigeons
    Soike, D
  • Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons
    Mankertz, A; Hattermann, K; Ehlers, B; Soike, D
  • Genetic diversity of pigeon circovirus in Hungary
    Csagola, A; Lorincz, M; Tombacz, K; Wladar, Z; Kovacs, E; Tuboly, T
  • ORF3 of duck circovirus: a novel protein with apoptotic activity
    Xiang, QW; Wang, X; Xie, ZJ; Sun, YN; Zhu, YL; Wang, SJ; Liu, HJ; Jiang, SJ
  • A method to diagnose Pigeon circovirus infection in vivo
    Hattermann, K; Soike, D; Grund, C; Mankertz, A
  • Rapid detection of pigeon herpesvirus, fowl adenovirus and pigeon circovirus in young racing pigeons by multiplex PCR
    Freick, M; Muller, H; Raue, R
  • Detection of a novel circovirus in mute swans ( Cygnus olor ) by using nested broad-spectrum PCR
    Halami, MY; Nieper, H; Muller, H; Johne, R

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