Epidemiological and genetic analysis of human group C rotaviruses isolated from outbreaks of acute gastroenteritis in Yokohama, Japan, between 2006 and 2012

Epidemiological and genetic analysis of human group C rotaviruses isolated from outbreaks of... Group C rotavirus (GCRV) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. Little is known about the yearly changes in the GCRV strains from diarrheal outbreaks. Stool samples collected from outbreaks of acute gastroenteritis in Yokohama, Japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group A rotavirus, were screened for GCRV using a reverse passive hemagglutination method. The GCRV strains were characterized by nucleotide sequence and phylogenetic analysis of their VP6, VP7, VP4, and NSP4 genes. Samples from nine of 735 outbreaks in Yokohama (1 %) contained GCRV, and eight of these outbreaks occurred in primary schools. The nucleotide sequences of the strains detected in this study were more closely related to Asian strains than to those from other regions of the world. The nucleotide sequences of the VP7 gene in these nine strains differed, and yearly changes were observed in the amino acid sequences of the VP4 genes. Phylogenetic trees constructed using the nucleotide sequences of the VP6, VP7, VP4, and NSP4 genes showed that sublineage S1 has divided into S1-1 and S1-2 in the VP4 gene only. Our results confirm that the prevalent strains of GCRV change yearly in Yokohama. This is the first study to demonstrate GCRV-associated gastroenteritis outbreaks in Yokohama, Japan. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Epidemiological and genetic analysis of human group C rotaviruses isolated from outbreaks of acute gastroenteritis in Yokohama, Japan, between 2006 and 2012

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1894-1
Publisher site
See Article on Publisher Site

Abstract

Group C rotavirus (GCRV) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. Little is known about the yearly changes in the GCRV strains from diarrheal outbreaks. Stool samples collected from outbreaks of acute gastroenteritis in Yokohama, Japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group A rotavirus, were screened for GCRV using a reverse passive hemagglutination method. The GCRV strains were characterized by nucleotide sequence and phylogenetic analysis of their VP6, VP7, VP4, and NSP4 genes. Samples from nine of 735 outbreaks in Yokohama (1 %) contained GCRV, and eight of these outbreaks occurred in primary schools. The nucleotide sequences of the strains detected in this study were more closely related to Asian strains than to those from other regions of the world. The nucleotide sequences of the VP7 gene in these nine strains differed, and yearly changes were observed in the amino acid sequences of the VP4 genes. Phylogenetic trees constructed using the nucleotide sequences of the VP6, VP7, VP4, and NSP4 genes showed that sublineage S1 has divided into S1-1 and S1-2 in the VP4 gene only. Our results confirm that the prevalent strains of GCRV change yearly in Yokohama. This is the first study to demonstrate GCRV-associated gastroenteritis outbreaks in Yokohama, Japan.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2014

References

  • The rhesus rotavirus VP4 sialic acid binding domain has a galectin fold with a novel carbohydrate binding site
    Dormitzer, PR; Sun, ZY; Wagner, G; Harrison, SC
  • Detection and molecular characterization of porcine group C rotaviruses in South Korea
    Jeong, YJ; Park, SI; Hosmillo, M; Shin, DJ; Chun, YH; Kim, HJ; Kwon, HJ; Kang, SY; Woo, SK; Park, SJ; Kim, GY; Kang, MI; Cho, KO
  • Genetic characterization of group C rotavirus isolated from a child hospitalized with acute gastroenteritis in Chiang Mai, Thailand
    Khamrin, P; Peerakome, S; Malasao, R; Mizuguchi, M; Okitsu, S; Ushijima, H; Maneekarn, N
  • Molecular characterization of a human group C rotavirus detected first in Turkey
    Mitui, MT; Bozdayi, G; Dalgic, B; Bostanci, I; Nishizono, A; Ahmed, K
  • First detection of group C rotavirus in children with acute gastroenteritis in South Korea
    Moon, S; Humphrey, CD; Kim, JS; Baek, LJ; Song, JW; Song, KJ; Jiang, B
  • First detection of group C rotavirus in children with acute diarrhea in Spain
    Sánchez-Fauquier, A; Roman, E; Colomina, J; Wilhelmi, I; Glass, RI; Jiang, B
  • Detection of group C rotaviruses in Tokyo
    Ushijima, H; Honma, H; Mukoyama, A; Shinozaki, T; Fujita, Y; Kobayashi, M; Ohseto, M; Morikawa, S; Kitamura, T

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