Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Enzymatic Lysis of Living Microbial Cells: A Universal Approach to Calculating the Rate of Cell Lysis in Turbidimetric Measurements

Enzymatic Lysis of Living Microbial Cells: A Universal Approach to Calculating the Rate of Cell... The data of the turbidimetric measurement for the enzymatic lysis of various living bacterial cells are analyzed. A method for the correct recalculation of the turbidimetric data (–ΔA/Δt) into absolute values of the change in the concentration of living cells (–ΔCFU/Δt) is proposed. The dimensionless efficiency of cell lysis–(1/CFU0) · ΔCFU/Δt for various bacterial cells is calculated to correctly compare the efficiency of the action of different bacteriolytic factors on various bacterial cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Chemistry Bulletin Springer Journals

Enzymatic Lysis of Living Microbial Cells: A Universal Approach to Calculating the Rate of Cell Lysis in Turbidimetric Measurements

Download PDF
6 pages

Loading next page...
 
/lp/springer_journal/enzymatic-lysis-of-living-microbial-cells-a-universal-approach-to-dpBbZqXYgY
Publisher
Springer Journals
Copyright
Copyright © 2018 by Allerton Press, Inc.
Subject
Chemistry; Chemistry/Food Science, general
ISSN
0027-1314
eISSN
1935-0260
DOI
10.3103/S0027131418020104
Publisher site
See Article on Publisher Site

Abstract

The data of the turbidimetric measurement for the enzymatic lysis of various living bacterial cells are analyzed. A method for the correct recalculation of the turbidimetric data (–ΔA/Δt) into absolute values of the change in the concentration of living cells (–ΔCFU/Δt) is proposed. The dimensionless efficiency of cell lysis–(1/CFU0) · ΔCFU/Δt for various bacterial cells is calculated to correctly compare the efficiency of the action of different bacteriolytic factors on various bacterial cells.

Journal

Moscow University Chemistry Bulletin – Springer Journals

Published: May 30, 2018

References

  • Molecular Cloning: A Laboratory Manual
    Maniatis, T.; Fritsch, E.F.; Sambrook, J.