Environmental chitinous materials as adsorbents for one-step purification of protease and chitosanase

Environmental chitinous materials as adsorbents for one-step purification of protease and... We describe the use of chitinous materials as adsorbents for purification of protease and chitosanase from bromelain solution and chitosanase from culture supernatants of three bacterial strains: Serratia marcescens TKU011, Bacillus cereus TKU022 and Acinetobacter calcoaceticus TKU024. The best adsorption results were observed when crude shrimp shell chitin (CSSC) and 750-nm chitin nanoparticles (CNP) were used. The optimum temperatures for protease adsorption from bromelain solution (22.9 mg/mL) by CSSC (0.1 g) and by 750-nm CNP (0.1 g) were 4 and 25 °C, respectively. The purification folds of bromelain by CSSC and 750-nm CNP were 5.2 and 4.5, respectively. For purification of protease from culture supernatants of TKU011, 750-nm CNP was 4.0-fold better than CSSC. However, CSSC exhibited purification folds of 2.9 and 3.3 for the chitosanases from TKU022 and TKU024, respectively. The adsorbed chitinolytic enzymes TKU015 chitinase (30 kDa) and TKU024 chitosanase (27 kDa) exhibited high purity by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Thus, CSSC and 750-nm CNP indicate potential for use as tools for one-step purification of bacterial chitinolytic enzymes from culture supernatants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Environmental chitinous materials as adsorbents for one-step purification of protease and chitosanase

Loading next page...
Springer Netherlands
Copyright © 2014 by Springer Science+Business Media Dordrecht
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial