Enhanced matrix metalloproteinase expression by Tisseel in mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts

Enhanced matrix metalloproteinase expression by Tisseel in mesothelial cells, normal peritoneal... Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) are important in regulating tissue remodeling of extracellular matrix. Dysregulation of MMPs is considered to be important to postoperative adhesion development. To examine whether Tisseel, a fibrin sealant, exerts an effect on expression of MMPs from human peritoneal mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts, a tissue culture study was conducted for 6, 12, 24, and 48 h under normoxic conditions. A multiplex reverse transcriptase–polymerase chain reaction technique using β-actin as a housekeeping gene was used to determine relative change in MMP-1, MMP-2, and TIMP-1 mRNA levels under six conditions (five fibrin sealant preparations and a control). Our results demonstrate that MMP-1, MMP-2, and TIMP-1 are all expressed by human peritoneal mesothelial cells and fibroblasts. All fibrin sealant compositions increased MMP-1 mRNA levels by all cell types, although the time course and pattern varied. The fibrin sealant compositions had variable effects on MMP-2 mRNA expression. Interestingly, in mesothelial cells, TIMP-1 mRNA levels were reduced by fibrin-sealant-containing aprotinin at both concentrations, while being further reduced by fibrin sealant without aprotinin, particularly at 6 and 12 h. In adhesion fibroblasts, there was generally no effect, while in normal fibroblasts, expression of TIMP-1 increased after 24 h. These results suggest that fibrin sealant, in addition to assisting in the achievement of hemostasis, exerts biological effects on human peritoneal mesothelial cells and fibroblasts and modulates MMP expression. Thus, fibrin sealant may influence tissue remodeling and may thereby be able to alter postoperative adhesion development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plastic Surgery Springer Journals

Enhanced matrix metalloproteinase expression by Tisseel in mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts

Loading next page...
 
/lp/springer_journal/enhanced-matrix-metalloproteinase-expression-by-tisseel-in-mesothelial-tS7z31fHfb
Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Medicine & Public Health; Plastic Surgery
ISSN
0930-343X
eISSN
1435-0130
D.O.I.
10.1007/s00238-005-0006-1
Publisher site
See Article on Publisher Site

Abstract

Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) are important in regulating tissue remodeling of extracellular matrix. Dysregulation of MMPs is considered to be important to postoperative adhesion development. To examine whether Tisseel, a fibrin sealant, exerts an effect on expression of MMPs from human peritoneal mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts, a tissue culture study was conducted for 6, 12, 24, and 48 h under normoxic conditions. A multiplex reverse transcriptase–polymerase chain reaction technique using β-actin as a housekeeping gene was used to determine relative change in MMP-1, MMP-2, and TIMP-1 mRNA levels under six conditions (five fibrin sealant preparations and a control). Our results demonstrate that MMP-1, MMP-2, and TIMP-1 are all expressed by human peritoneal mesothelial cells and fibroblasts. All fibrin sealant compositions increased MMP-1 mRNA levels by all cell types, although the time course and pattern varied. The fibrin sealant compositions had variable effects on MMP-2 mRNA expression. Interestingly, in mesothelial cells, TIMP-1 mRNA levels were reduced by fibrin-sealant-containing aprotinin at both concentrations, while being further reduced by fibrin sealant without aprotinin, particularly at 6 and 12 h. In adhesion fibroblasts, there was generally no effect, while in normal fibroblasts, expression of TIMP-1 increased after 24 h. These results suggest that fibrin sealant, in addition to assisting in the achievement of hemostasis, exerts biological effects on human peritoneal mesothelial cells and fibroblasts and modulates MMP expression. Thus, fibrin sealant may influence tissue remodeling and may thereby be able to alter postoperative adhesion development.

Journal

European Journal of Plastic SurgerySpringer Journals

Published: Feb 1, 2006

References

  • Evaluation of resorbable barriers for preventing surgical adhesions
    Arnold, PB; Green, CW; Foresman, PA; Rodeheaver, GT
  • Structural properties of matrix metalloproteinases
    Bode, WC; Fernadez-Catalan, H; Tschesche, H; Grams, F; Nagase, H; Maskos, K

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off