Enhanced diterpene lactone (andrographolide)
production from elicited adventitious root cultures
of Andrographis paniculata
Charu Chandra Giri
Received: 11 August 2016 / Accepted: 8 October 2016 / Published online: 20 October 2016
Ó Springer Science+Business Media Dordrecht 2016
Abstract The inﬂuence of different chemical elicitors was evaluated for increased
andrographolide production using adventitious root cultures of Andrographis pan-
iculata. Fresh weight (FW) and dry weight (DW) of adventitious roots was
decreased with increased concentrations of all the elicitors (Jasmonic acid: JA;
Salicylic acid: SA; Acetyl salicylic acid: ASA and Methyl salicylic acid: MeSA).
However, especially with 1 lM JA, an increase of FW and DW was observed in all
weeks, except the fourth and seventh weeks, when compared to un-elicited (control)
cultures. JA concentration at 1 lM was effective and 3.5-fold improvement in
andrographolide production over control cultures was observed after the ﬁrst week.
Maximum andrographolide content of 10.8-fold (2.548 % DW) was obtained after
the ﬁrst week using adventitious root cultures elicited with 25 lM JA compared to
control (0.234 % DW). Amongst the concentrations of SA and its derivatives,
elicitation with MeSA at 100 lM for seven days promoted 2.6-fold increase in
andrographolide production. JA was found superior than SA, and JA stimulated
increased andrographolide content in adventitious root cultures of A. paniculata.
Our observations indicated that an inoculum (0.02 g) of adventitious roots can
generate as much as a 1200 fold increased biomass (25 g) within twenty weeks by
repeated multiplication with a subculture interval of four weeks. These results may
be helpful for large-scale cultivation of adventitious root cultures and production of
pharmaceutically important andrographolide. This is the ﬁrst report on elicitation
using adventitious root cultures of A. paniculata involving chemical elicitors.
Keywords Adventitious root Á Andrographis paniculata Á Andrographolides Á
Biomass growth Á Elicitation Á Yield enhancement
& Charu Chandra Giri
Centre for Plant Molecular Biology (CPMB), Osmania University, Hyderabad,
Telangana 500007, India
Res Chem Intermed (2017) 43:2433–2444