Access the full text.
Sign up today, get DeepDyve free for 14 days.
E. Goodpasture, F. Talbot (1921)
CONCERNING THE NATURE OF PROTOZOAN-LIKE CELLS IN CERTAIN LESIONS OF INFANCYJAMA Pediatrics, 21
K. Kigasawa, S. Soushi, Y. Tanaka, H. Obazawa (1994)
Morphologic and chromosomal study of a human retinal pigment epithelial cell line.Japanese journal of ophthalmology, 38 1
E. Gonczol, P. Andrews, S. Plotkin (1984)
Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells.Science, 224 4645
D. Wolff, C. Sinzger, P. Drescher, G. Jahn, B. Plachter (1994)
Reduced levels of IE2 gene expression and shutdown of early and late viral genes during latent infection of the glioblastoma cell line U138-MG with selectable recombinants of human cytomegalovirus.Virology, 204 1
P. Wallace, W. Janet, W. Samuel, C. Horace, J. Robert (1956)
Cytopathogenic Agent Resembling Human Salivary Gland Virus Recovered from Tissue Cultures of Human AdenoidsProceedings of the Society for Experimental Biology and Medicine, 92
M. Boshart, F. Weber, G. Jahn, Karoline Dorsch-H⇒ler, B. Fleckenstein, W. Schaffner (1985)
A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirusCell, 41
S. Michelson-Fiske, J. Arnoult, H. Febvre (1975)
Cytomegalovirus infection of human lung epithelial cells in vitro.Intervirology, 5 6
Christian Sinzger, Annemarie Grefte, B. Plachter, Annette Gouw, Gerhard Jahn (1995)
Fibroblasts, epithelial cells, endothelial cells and smooth muscle cells are major targets of human cytomegalovirus infection in lung and gastrointestinal tissues.The Journal of general virology, 76 ( Pt 4)
B. Detrick, Jean Rhame, Yun Wang, C. Nagineni, J. Hooks (1996)
Cytomegalovirus replication in human retinal pigment epithelial cells. Altered expression of viral early proteins.Investigative ophthalmology & visual science, 37 5
T. Cha, E. Tom, G. Kemble, G. Duke, E. Mocarski, R. Spaete (1996)
Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strainsJournal of Virology, 70
K. Yamanishi, F. Rapp (1979)
Induction of host DNA synthesis and DNA polymerase by DNA-negative temperature-sensitive mutants of human cytomegalovirus.Virology, 94 1
S. Tugizov, E. Maidji, L. Pereira (1996)
Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells.The Journal of general virology, 77 ( Pt 1)
A. Angulo, C. Suto, M. Boehm, R. Heyman, P. Ghazal (1995)
Retinoid activation of retinoic acid receptors but not of retinoid X receptors promotes cellular differentiation and replication of human cytomegalovirus in embryonal cellsJournal of Virology, 69
P. Rosen, S. Hajdu (1971)
Cytomegalovirus inclusion disease at autopsy of patients with cancer.American journal of clinical pathology, 55 6
B. Seed (1987)
An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2Nature, 329
K. Numazaki, H. Goldman, I. Wong, M. Wainberg (1988)
Viral infection of human fetal islets of Langerhans. Replication of human cytomegalovirus in cultured human fetal pancreatic islets.American journal of clinical pathology, 90 1
J. Demarchi, A. Kaplan (1977)
Physiological State of Human Embryonic Lung Cells Affects Their Response to Human CytomegalovirusJournal of Virology, 23
T. Weller (1971)
The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. I.The New England journal of medicine, 285 4
(1934)
The problem of intranuclear inclusions in virus diseases
T. Iwasaki (1987)
ALIMENTARY TRACT LESIONS IN CYTOMEGALOVIRUS INFECTIONPathology International, 37
M. Heieren, Youngki Kim, H. Balfour (1988)
Human cytomegalovirus infection of kidney glomerular visceral epithelial and tubular epithelial cells in culture.Transplantation, 46 3
Margaret Smith (1956)
Propagation in Tissue Cultures of a Cytopathogenic Virus from Human Salivary Gland Virus (SGV) Disease.∗Proceedings of the Society for Experimental Biology and Medicine, 92
E. Mocarski, M. Bonyhadi, S. Salimi, Joseph McCune, H. Kaneshima (1993)
Human cytomegalovirus in a SCID-hu mouse: thymic epithelial cells are prominent targets of viral replication.Proceedings of the National Academy of Sciences of the United States of America, 90 1
M. Ho (1982)
Cytomegalovirus: Biology and Infection
M. Miceli, D. Newsome, L. Novak, R. Beuerman (1989)
Cytomegalovirus replication in cultured human retinal pigment epithelial cells.Current eye research, 8 8
D. Thomsen, R. Stenberg, W. Goins, M. Stinski (1984)
Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.Proceedings of the National Academy of Sciences of the United States of America, 81 3
T. Weller, J. Macauley, J. Craig, P. Wirth (1957)
Isolation of Intranuclear Inclusion Producing Agents from Infants with Illnesses Resembling Cytomegalic Inclusion Disease.∗ †Proceedings of the Society for Experimental Biology and Medicine, 94
Although human cytomegalovirus (HCMV) predominantly infects epithelial cells in vivo, the majority of studies of HCMV gene expression and replication have been conducted using non-epithelial cell lines in part because of the absence of a good experimental system using epithelial cells. To address the nature of epithelial cell infection, we investigated the susceptibility of an epithelial cell line (K-1034) established from the retinal pigment epithelium to HCMV infection. This cell line exhibited high susceptibility to HCMV, as evidenced by detection of one of the immediate early antigens, IE2, in the nuclei of more than 80% of K-1034 cells at 24 h following inoculation at a multiplicity of infection of 3 plaque forming units per cell. However, the yield after one-step growth of HCMV in K-1034 cells was about twenty-fold less than that in human embryonic lung fibroblast cells. Cytopathic effect (CPE) on K-1034 cells was not prominent in medium supplemented with 10% fetal bovine serum and viral late antigens were detected in less than 5% of K-1034 cells. Interestingly, infected cells expressing late antigens and exhibiting CPE were markedly increased in serum-free medium, even though the yield of infectious HCMV and viral genome copy numbers were almost the same in the different serum concentrations, due to viral instability in the absence of serum. Thus, the progression of late antigens expression and the induction of CPE in infected epithelial cells is influenced by physiological conditions, and are negatively regulated by some serum factor.
Archives of Virology – Springer Journals
Published: Aug 1, 1997
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.