Enhanced core protein production by hepatitis B virus bearing a mutation in the precore region

Enhanced core protein production by hepatitis B virus bearing a mutation in the precore region An HBV DNA tandem dimer bearing GTG instead of the precore initiation codon (p2WPC-), that bearing an amber mutation at precore codon 28 (p2WPCTer) and that of a wild-type were introduced into HepG2 cells. p2WPC- produced no HBeAg, the same amount of HBsAg as the wild-type, and 2–4 times as much HBcAg and progeny virus DNA. p2WPCTer produced no HBeAg, the same amount of HBsAg, 2 times as much HBcAg and a slightly increased amount of progeny virus. The amounts of p21 c peptide in both mutants determined by immunoblotting correlated well with the ELISA titers. These results suggest that these precore single point mutations are responsible for the enhanced core peptide production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Enhanced core protein production by hepatitis B virus bearing a mutation in the precore region

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Publisher
Springer Journals
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050158
Publisher site
See Article on Publisher Site

Abstract

An HBV DNA tandem dimer bearing GTG instead of the precore initiation codon (p2WPC-), that bearing an amber mutation at precore codon 28 (p2WPCTer) and that of a wild-type were introduced into HepG2 cells. p2WPC- produced no HBeAg, the same amount of HBsAg as the wild-type, and 2–4 times as much HBcAg and progeny virus DNA. p2WPCTer produced no HBeAg, the same amount of HBsAg, 2 times as much HBcAg and a slightly increased amount of progeny virus. The amounts of p21 c peptide in both mutants determined by immunoblotting correlated well with the ELISA titers. These results suggest that these precore single point mutations are responsible for the enhanced core peptide production.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 1997

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