Engineered retroviral virus-like particles for receptor targeting

Engineered retroviral virus-like particles for receptor targeting Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). This occurs in mammalian and bacterial cells as well as in in vitro systems. In every circumstance, nucleic acids are incorporated into the forming particles. Here, we took advantage of an in vitro system for the generation of non-enveloped Mason-Pfizer monkey virus (M-PMV) VLPs derived from a self-assembling CA-NC subunit of Gag. These VLPs were modified through N-terminal extension of CA-NC with short oligopeptides that, after the assembly process, were exposed on the surface of VLPs. The employed N-terminal modifications allowed specific interaction with target cells expressing prostate-specific membrane antigen. Using this system, we were able to incorporate selected siRNA into forming VLPs and deliver it into the cytosol of target cells. In comparison with other viral vectors designed for targeted transgene delivery, this M-PMV VLP system represents the lowest risk of generating virus-associated pathology, as the VLPs do not contain any viral coding sequences and are formed in a cell-free system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Engineered retroviral virus-like particles for receptor targeting

Loading next page...
 
/lp/springer_journal/engineered-retroviral-virus-like-particles-for-receptor-targeting-thlk5d0WXB
Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1873-6
Publisher site
See Article on Publisher Site

Abstract

Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). This occurs in mammalian and bacterial cells as well as in in vitro systems. In every circumstance, nucleic acids are incorporated into the forming particles. Here, we took advantage of an in vitro system for the generation of non-enveloped Mason-Pfizer monkey virus (M-PMV) VLPs derived from a self-assembling CA-NC subunit of Gag. These VLPs were modified through N-terminal extension of CA-NC with short oligopeptides that, after the assembly process, were exposed on the surface of VLPs. The employed N-terminal modifications allowed specific interaction with target cells expressing prostate-specific membrane antigen. Using this system, we were able to incorporate selected siRNA into forming VLPs and deliver it into the cytosol of target cells. In comparison with other viral vectors designed for targeted transgene delivery, this M-PMV VLP system represents the lowest risk of generating virus-associated pathology, as the VLPs do not contain any viral coding sequences and are formed in a cell-free system.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2014

References

  • Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy
    Bharat, TA; Davey, NE; Ulbrich, P; Riches, JD; Marco, A; Rumlova, M; Sachse, C; Ruml, T; Briggs, JA
  • Viral vectors: from virology to transgene expression
    Bouard, D; Alazard-Dany, D; Cosset, FL
  • Solution structure and dynamics of the Rous sarcoma virus capsid protein and comparison with capsid proteins of other retroviruses
    Campos-Olivas, R; Newman, JL; Summers, MF
  • Gene delivery by lentivirus vectors
    Cockrell, AS; Kafri, T
  • From the first to the third generation adenoviral vector: what parameters are governing the production yield?
    Dormond, E; Perrier, M; Kamen, A
  • Changing the surface of a virus shell fusion of an enzyme to polyoma VP1
    Gleiter, S; Stubenrauch, K; Lilie, H
  • Coupling of antibodies via protein Z on modified polyoma virus-like particles
    Gleiter, S; Lilie, H
  • A HA2-Fusion tag limits the endosomal release of its protein cargo despite causing endosomal lysis
    Lee, YJ; Johnson, G; Peltier, GC; Pellois, JP
  • Latest development in viral vectors for gene therapy
    Lundstrom, K
  • Retroviral vectors for human gene delivery
    McTaggart, S; Al-Rubeai, M
  • Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer
    Raper, SE; Chirmule, N; Lee, FS; Wivel, NA; Bagg, A; Gao, GP; Wilson, JM; Batshaw, ML
  • Gene therapy: twenty-first century medicine
    Verma, IM; Weitzman, MD
  • Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography
    Vorackova, I; Suchanova, S; Ulbrich, P; Diehl, WE; Ruml, T
  • A cationic lipid emulsion/DNA complex as a physically stable and serum-resistant gene delivery system
    Yi, SW; Yune, TY; Kim, TW; Chung, H; Choi, YW; Kwon, IC; Lee, EB; Jeong, SY

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off