Engineered retroviral virus-like particles for receptor targeting

Engineered retroviral virus-like particles for receptor targeting Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). This occurs in mammalian and bacterial cells as well as in in vitro systems. In every circumstance, nucleic acids are incorporated into the forming particles. Here, we took advantage of an in vitro system for the generation of non-enveloped Mason-Pfizer monkey virus (M-PMV) VLPs derived from a self-assembling CA-NC subunit of Gag. These VLPs were modified through N-terminal extension of CA-NC with short oligopeptides that, after the assembly process, were exposed on the surface of VLPs. The employed N-terminal modifications allowed specific interaction with target cells expressing prostate-specific membrane antigen. Using this system, we were able to incorporate selected siRNA into forming VLPs and deliver it into the cytosol of target cells. In comparison with other viral vectors designed for targeted transgene delivery, this M-PMV VLP system represents the lowest risk of generating virus-associated pathology, as the VLPs do not contain any viral coding sequences and are formed in a cell-free system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Engineered retroviral virus-like particles for receptor targeting

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1873-6
Publisher site
See Article on Publisher Site

References

  • Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy
    Bharat, TA; Davey, NE; Ulbrich, P; Riches, JD; Marco, A; Rumlova, M; Sachse, C; Ruml, T; Briggs, JA
  • Viral vectors: from virology to transgene expression
    Bouard, D; Alazard-Dany, D; Cosset, FL
  • Solution structure and dynamics of the Rous sarcoma virus capsid protein and comparison with capsid proteins of other retroviruses
    Campos-Olivas, R; Newman, JL; Summers, MF
  • Gene delivery by lentivirus vectors
    Cockrell, AS; Kafri, T
  • From the first to the third generation adenoviral vector: what parameters are governing the production yield?
    Dormond, E; Perrier, M; Kamen, A
  • Changing the surface of a virus shell fusion of an enzyme to polyoma VP1
    Gleiter, S; Stubenrauch, K; Lilie, H
  • Coupling of antibodies via protein Z on modified polyoma virus-like particles
    Gleiter, S; Lilie, H
  • A HA2-Fusion tag limits the endosomal release of its protein cargo despite causing endosomal lysis
    Lee, YJ; Johnson, G; Peltier, GC; Pellois, JP
  • Latest development in viral vectors for gene therapy
    Lundstrom, K
  • Retroviral vectors for human gene delivery
    McTaggart, S; Al-Rubeai, M
  • Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer
    Raper, SE; Chirmule, N; Lee, FS; Wivel, NA; Bagg, A; Gao, GP; Wilson, JM; Batshaw, ML
  • Gene therapy: twenty-first century medicine
    Verma, IM; Weitzman, MD
  • Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography
    Vorackova, I; Suchanova, S; Ulbrich, P; Diehl, WE; Ruml, T
  • A cationic lipid emulsion/DNA complex as a physically stable and serum-resistant gene delivery system
    Yi, SW; Yune, TY; Kim, TW; Chung, H; Choi, YW; Kwon, IC; Lee, EB; Jeong, SY

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