Energy exchange and ultrastructure of plant cells under generation of nitric oxide

Energy exchange and ultrastructure of plant cells under generation of nitric oxide The effects of nitric oxide (NO) on oxygen consumption, heat generation, and cell ultrastructure were investigated in the seedlings of wheat (Triticum aestivum L.). The experiments were conducted with the excised roots of 5-day-old seedlings grown in the solution of CaCl2 (2.5 × 10−4 M). The source of NO was NaNO2 (5 × 10−3 M) where the roots were incubated. Production of NO was determined by means of EPR, respiration — gasometrically, heat generation — using a microcalorimeter. The results showed that NO was formed in the presence of NaNO2. This was accompanied by a decrease in the respiration rate by about 30%, which lasted for 5–6 h. Apparently, NO inhibited mitochondrial oxidation because stimulation of oxygen consumption induced by 2,4-DNP was completely removed in the presence of NaNO2. When the cells were affected by succinic acid in the presence of NaNO2, respiration was strongly inhibited. The effects of succinic acid and NaNO2 were negated by ascorbic acid. A decrease in the rate of respiration was accompanied by a reduction in heat generation. Moreover, the efflux of potassium ions to the root incubation medium was stimulated, which may point to changes in ionic membrane permeability. The observed changes in energy exchange were accompanied by disturbances in the cell ultrastructure. Nitric oxide induced a clarification of the mitochondrial matrix and a reduction in the number of cristae. It was concluded that NO excess in plant tissues brings about a deceleration of energy exchange, disturbance of the ultrastructural organization, and cell death. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Energy exchange and ultrastructure of plant cells under generation of nitric oxide

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710061044
Publisher site
See Article on Publisher Site

Abstract

The effects of nitric oxide (NO) on oxygen consumption, heat generation, and cell ultrastructure were investigated in the seedlings of wheat (Triticum aestivum L.). The experiments were conducted with the excised roots of 5-day-old seedlings grown in the solution of CaCl2 (2.5 × 10−4 M). The source of NO was NaNO2 (5 × 10−3 M) where the roots were incubated. Production of NO was determined by means of EPR, respiration — gasometrically, heat generation — using a microcalorimeter. The results showed that NO was formed in the presence of NaNO2. This was accompanied by a decrease in the respiration rate by about 30%, which lasted for 5–6 h. Apparently, NO inhibited mitochondrial oxidation because stimulation of oxygen consumption induced by 2,4-DNP was completely removed in the presence of NaNO2. When the cells were affected by succinic acid in the presence of NaNO2, respiration was strongly inhibited. The effects of succinic acid and NaNO2 were negated by ascorbic acid. A decrease in the rate of respiration was accompanied by a reduction in heat generation. Moreover, the efflux of potassium ions to the root incubation medium was stimulated, which may point to changes in ionic membrane permeability. The observed changes in energy exchange were accompanied by disturbances in the cell ultrastructure. Nitric oxide induced a clarification of the mitochondrial matrix and a reduction in the number of cristae. It was concluded that NO excess in plant tissues brings about a deceleration of energy exchange, disturbance of the ultrastructural organization, and cell death.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jan 8, 2011

References

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