J. Membrane Biol. 170, 1–12 (1999) The Journal of Membrane Biology © Springer-Verlag New York Inc. 1999 Topical Review W.-M. Weber Laboratory of Physiology, K. U. Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium Received: 20 October 1998/Revised: 3 April 1999 Introduction Oocytes of Xenopus laevis are huge cells with a diameter ranging from 1 to 1.3 mm. Their size allows Xenopus laevis oocytes devoid of their follicular enclo- simple handling with ordinary Pasteur pipettes whose sure provide a frequently used expression system for sharp edges have been fire polished. Moreover, the size investigating channels, transporters and receptors follow- of the oocytes permits the combination of several tech- ing injection of the appropriate mRNA or cDNA ex- niques on a single cell. So is it rather easy to inject tracted from a wide variety of tissues including plants substances into the oocyte while the membrane potential (elegantly reviewed by Sigel ). However, if one is monitored or while being voltage-clamped . The intends to make use of the oocyte expression system, it is experimenter could have access to intracellular changes absolutely imperative to know whether or not the oocyte during electrophysiological experiments if it is required endogenously already possesses the transport
The Journal of Membrane Biology – Springer Journals
Published: Jul 1, 1999
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