Epigallocatechin-3-gallate (EGCG), an eco-friendly polyphenol, has a strong inhibitory effect on the bloom-forming cyanobacterium Microcystis aeruginosa. In order to reveal the molecular mechanism of algal inhibition of plant polyphenols, quantitative proteome analysis based on iTRAQ (isobaric tags for relative and absolute quantification) was applied to investigate EGCG-induced proteomic changes in M. aeruginosa. Following treatment with EGCG for 48 h, the total protein content was compared with that of untreated cells, and 88 differentially expressed proteins were identified, of which 30 were upregulated and 58 were downregulated. Proteins involved in chlorophyll biosynthesis, carbon and nitrate assimilation and cell division were among the most downregulated, which resulted in growth suppression. By contrast, anti-oxidative proteins and molecular chaperones, such as superoxide dismutase, glutaredoxin, and heat shock proteins, were distinctly upregulated. Eighteen potentially crucial proteins were selected for assessment of transcription by real-time quantitative PCR, which confirmed the results of proteomic experiment. In addition, immunoblotting confirmed downregulation of three representative proteins, magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase AcsF, glutamine synthetase GlnN, and metalloprotease FtsH, following EGCG treatment, consistent with the results of iTRAQ quantitation experiment. It is speculated that chlorophyll biosynthesis, carbon and nitrate assimilation, and cell division are the main inhibition targets of EGCG.
Journal of Applied Phycology – Springer Journals
Published: May 11, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud