Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency of Agrobacterium-mediated transformation in Petunia

Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency... Physiol Mol Biol Plants https://doi.org/10.1007/s12298-018-0553-0 RESEARCH ARTICLE Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency of Agrobacterium-mediated transformation in Petunia 1 2 3 • • Aso Nobakht Vakili Hedayat Bagheri Pejman Azadi Received: 25 October 2017 / Revised: 5 May 2018 / Accepted: 14 May 2018 Prof. H.S. Srivastava Foundation for Science and Society 2018 Abstract In order to evaluate the effect of inoculation and Keywords Macro elements  Co-cultivation co-cultivation media elements on transformation frequency Agrobacterium tumefaciens  Transformation  Petunia in Petunia hybrida, modified MS media with different hybrida elements were tested on Alvan and Large Flower Alvan (LF Alvan), two local cultivars. Leaf explants of both Abbreviations cultivars were inoculated with Agrobacterium tumefaciens LF Alvan Large Flower Alvan strain LBA4404 (pBI121) containing neomycin phospho- GUS b-glucuronidase transferase (nptII) and an intron-containing b-glu- CTAB Cetyltrimethylammonium bromide curonidase (gus) genes. When medium lacking KH PO , nptII Neomycin phosphotransferase 2 4 NH NO , KNO , and CaCl was used as inoculation and CaMV Cauliflower mosaic virus 4 3 3 2 co-cultivation medium, a higher frequency of transforma- tion for Alvan (22%) and LF Alvan (16%) was obtained. Kanamycin resistant plantlets were stained blue http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Physiology and Molecular Biology of Plants Springer Journals

Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency of Agrobacterium-mediated transformation in Petunia

Loading next page...
 
/lp/springer_journal/elimination-of-macro-elements-from-inoculation-and-co-cultivation-WTy79vdR8V
Publisher
Springer India
Copyright
Copyright © 2018 by Prof. H.S. Srivastava Foundation for Science and Society
Subject
Life Sciences; Plant Sciences; Plant Physiology; Biological and Medical Physics, Biophysics; Cell Biology
ISSN
0971-5894
eISSN
0974-0430
D.O.I.
10.1007/s12298-018-0553-0
Publisher site
See Article on Publisher Site

Abstract

Physiol Mol Biol Plants https://doi.org/10.1007/s12298-018-0553-0 RESEARCH ARTICLE Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency of Agrobacterium-mediated transformation in Petunia 1 2 3 • • Aso Nobakht Vakili Hedayat Bagheri Pejman Azadi Received: 25 October 2017 / Revised: 5 May 2018 / Accepted: 14 May 2018 Prof. H.S. Srivastava Foundation for Science and Society 2018 Abstract In order to evaluate the effect of inoculation and Keywords Macro elements  Co-cultivation co-cultivation media elements on transformation frequency Agrobacterium tumefaciens  Transformation  Petunia in Petunia hybrida, modified MS media with different hybrida elements were tested on Alvan and Large Flower Alvan (LF Alvan), two local cultivars. Leaf explants of both Abbreviations cultivars were inoculated with Agrobacterium tumefaciens LF Alvan Large Flower Alvan strain LBA4404 (pBI121) containing neomycin phospho- GUS b-glucuronidase transferase (nptII) and an intron-containing b-glu- CTAB Cetyltrimethylammonium bromide curonidase (gus) genes. When medium lacking KH PO , nptII Neomycin phosphotransferase 2 4 NH NO , KNO , and CaCl was used as inoculation and CaMV Cauliflower mosaic virus 4 3 3 2 co-cultivation medium, a higher frequency of transforma- tion for Alvan (22%) and LF Alvan (16%) was obtained. Kanamycin resistant plantlets were stained blue

Journal

Physiology and Molecular Biology of PlantsSpringer Journals

Published: May 31, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off