Physiol Mol Biol Plants https://doi.org/10.1007/s12298-018-0553-0 RESEARCH ARTICLE Elimination of macro elements from inoculation and co-cultivation media enhances the efﬁciency of Agrobacterium-mediated transformation in Petunia 1 2 3 • • Aso Nobakht Vakili Hedayat Bagheri Pejman Azadi Received: 25 October 2017 / Revised: 5 May 2018 / Accepted: 14 May 2018 Prof. H.S. Srivastava Foundation for Science and Society 2018 Abstract In order to evaluate the effect of inoculation and Keywords Macro elements Co-cultivation co-cultivation media elements on transformation frequency Agrobacterium tumefaciens Transformation Petunia in Petunia hybrida, modiﬁed MS media with different hybrida elements were tested on Alvan and Large Flower Alvan (LF Alvan), two local cultivars. Leaf explants of both Abbreviations cultivars were inoculated with Agrobacterium tumefaciens LF Alvan Large Flower Alvan strain LBA4404 (pBI121) containing neomycin phospho- GUS b-glucuronidase transferase (nptII) and an intron-containing b-glu- CTAB Cetyltrimethylammonium bromide curonidase (gus) genes. When medium lacking KH PO , nptII Neomycin phosphotransferase 2 4 NH NO , KNO , and CaCl was used as inoculation and CaMV Cauliﬂower mosaic virus 4 3 3 2 co-cultivation medium, a higher frequency of transforma- tion for Alvan (22%) and LF Alvan (16%) was obtained. Kanamycin resistant plantlets were stained blue
Physiology and Molecular Biology of Plants – Springer Journals
Published: May 31, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera