Elicitor mediated enhancement of wedelolactone in cell suspension culture of Eclipta alba (L.) Hassk

Elicitor mediated enhancement of wedelolactone in cell suspension culture of Eclipta alba (L.) Hassk The present research focused on enhancing the production of wedelolactone through cell suspension culture (CSC) in Eclipta alba (L.) Hassk. With an aim of attaining a sustainable CSC, various plant growth regulators, elicitors and agitation speed were examined. Nodal segments of in vitro propagated plantlets induced the maximum percentage (93.47 ± 0.61%) of −1 callus inoculated on Murashige and Skoog (MS) medium fortified with picloram (2 mg L ). The growth kinetics of CSC exhibited a sigmoid pattern with a lag phase (0–6 days), a log phase (6–18 days), a stationary phase (18–24 days) and then −1 death phase thereafter. The highest biomass accumulation in CSC with 7.09 ± 0.06 g 50 mL fresh weight, 1.52 ± 0.02 g −1 6 −1 50 mL dry cell weight, 1.34 ± 0.01 × 10  cell mL total cell count and 57.00 ± 0.58% packed cell volume was obtained −1 −1 in the liquid MS medium supplemented with 1.5 mg L picloram plus 0.5 mg L kinetin at 120 rpm. High performance −1 thin layer chromatography confirmed that yeast extract (biotic elicitor) at 150 mg L accumulated more CSC biomass −1 with 1.22-fold increase in wedelolactone (288.97 ± 1.94 µg g dry weight) content in comparison to the non-elicited CSC −1 (237.78 ± 0.04 µg g dry weight) after 120 h of incubation. Contrastingly, methyl jasmonate (abiotic elicitor) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

Elicitor mediated enhancement of wedelolactone in cell suspension culture of Eclipta alba (L.) Hassk

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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Plant Sciences; Plant Physiology; Plant Genetics and Genomics; Plant Pathology
ISSN
0167-6857
eISSN
1573-5044
D.O.I.
10.1007/s11240-018-1431-8
Publisher site
See Article on Publisher Site

Abstract

The present research focused on enhancing the production of wedelolactone through cell suspension culture (CSC) in Eclipta alba (L.) Hassk. With an aim of attaining a sustainable CSC, various plant growth regulators, elicitors and agitation speed were examined. Nodal segments of in vitro propagated plantlets induced the maximum percentage (93.47 ± 0.61%) of −1 callus inoculated on Murashige and Skoog (MS) medium fortified with picloram (2 mg L ). The growth kinetics of CSC exhibited a sigmoid pattern with a lag phase (0–6 days), a log phase (6–18 days), a stationary phase (18–24 days) and then −1 death phase thereafter. The highest biomass accumulation in CSC with 7.09 ± 0.06 g 50 mL fresh weight, 1.52 ± 0.02 g −1 6 −1 50 mL dry cell weight, 1.34 ± 0.01 × 10  cell mL total cell count and 57.00 ± 0.58% packed cell volume was obtained −1 −1 in the liquid MS medium supplemented with 1.5 mg L picloram plus 0.5 mg L kinetin at 120 rpm. High performance −1 thin layer chromatography confirmed that yeast extract (biotic elicitor) at 150 mg L accumulated more CSC biomass −1 with 1.22-fold increase in wedelolactone (288.97 ± 1.94 µg g dry weight) content in comparison to the non-elicited CSC −1 (237.78 ± 0.04 µg g dry weight) after 120 h of incubation. Contrastingly, methyl jasmonate (abiotic elicitor)

Journal

Plant Cell, Tissue and Organ CultureSpringer Journals

Published: May 31, 2018

References

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