Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium Synechocystis sp. PCC 6803

Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium... The trxA gene encoding one of the different thioredoxins of the facultative heterotrophic cyanobacterium Synechocystis sp. PCC 6803 is transcribed as a single mRNA of 450 nucleotides. Transcript accumulation is similar in all standard growth conditions but strongly decreases after transferring cell cultures from light to darkness. In steady-state conditions, trxA transcription is reduced at high (150–500 μE m−2 s−1) compared with moderate (10–50 μE m−2 s−1) light intensities. The stability of the trxA transcript was similar at different light intensities, and also in darkness. Photosynthetic electron transport inhibitors, as well as glucose starvation in a mutant strain lacking photosystem II, promote a strong decline in the level of trxA transcript. Primer extension analysis suggests that trxA is transcribed from two proximal promoters containing a −10 TATA box similar to the Escherichia coli consensus promoters. Unlike the trxA mRNA, the amount of thioredoxin protein was not reduced in the dark, neither at high light intensities, indicating that thioredoxin protein is very stable. Our results indicate that the thioredoxin encoded by the trxA gene is likely to be primarily regulated at the transcriptional level, rather than at the protein level, by the electron transport generated photosynthetically or from glucose metabolism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium Synechocystis sp. PCC 6803

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Publisher
Springer Journals
Copyright
Copyright © 2000 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006472018601
Publisher site
See Article on Publisher Site

Abstract

The trxA gene encoding one of the different thioredoxins of the facultative heterotrophic cyanobacterium Synechocystis sp. PCC 6803 is transcribed as a single mRNA of 450 nucleotides. Transcript accumulation is similar in all standard growth conditions but strongly decreases after transferring cell cultures from light to darkness. In steady-state conditions, trxA transcription is reduced at high (150–500 μE m−2 s−1) compared with moderate (10–50 μE m−2 s−1) light intensities. The stability of the trxA transcript was similar at different light intensities, and also in darkness. Photosynthetic electron transport inhibitors, as well as glucose starvation in a mutant strain lacking photosystem II, promote a strong decline in the level of trxA transcript. Primer extension analysis suggests that trxA is transcribed from two proximal promoters containing a −10 TATA box similar to the Escherichia coli consensus promoters. Unlike the trxA mRNA, the amount of thioredoxin protein was not reduced in the dark, neither at high light intensities, indicating that thioredoxin protein is very stable. Our results indicate that the thioredoxin encoded by the trxA gene is likely to be primarily regulated at the transcriptional level, rather than at the protein level, by the electron transport generated photosynthetically or from glucose metabolism.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 16, 2004

References

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