Res. Chem. Intermed.
, Vol. 32, No. 5–6, pp. 595–601 (2006)
Also available online - www.vsppub.com
Electrochemical detection of ascorbic acid and serotonin
at a boron-doped diamond electrode modiﬁed with
, JONG-EUN PARK
, EUN-IN CHO
, JIN-HEE HWANG
and TAKEO OHSAKA
Department of Industrial and Chemical Engineering, Chungbuk National University, San 48,
Geshindong, Cheong-ju, Chungbuk 361-763, South Korea
Graduate School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
Department of Electronic Chemistry, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku,
Yokohama 226-8502, Japan
Received 24 January 2005; accepted 28 February 2005
Abstract—The electrochemical oxidation of ascorbic acid (AA) and serotonin (5-HT) at a boron-
doped diamond (BDD) electrode modiﬁed with poly(N,N -dimethylaniline) (PDMA) has been
studied. The oxidation potentials of 5-HT and AA overlapped after mixing of the two chemicals,
due to interference of AA at the bare BDD electrode. However, after modifying the BDD electrode
with a cationic polymer (PDMA), the oxidation peaks of 5-HT and AA were separated. PDMA-coated
BDD electrodes can be used for simultaneous detection of these species.
Keywords: Electropolymerization; boron-doped diamond electrode; ascorbic acid; serotonin; square
Serotonin, also known as 5-hydroxytryptamine (5-HT), is produced from the amino-
acid tryptophan inside the human body . Once released from the serotonergic
neurons, 5-HT acts as a neurotransmitter, attaching to various 5-HT receptors.
5-HT is metabolized and inactivated by the enzymes monoamine oxidase and
aldehyde dehydrogenase . 5-HT deﬁciency has been implicated in various
medical disorders, including depression, migraine headaches, sleep disorders,
bulimia and premenstrual syndrome . Electrochemical detection of 5-HT has
received a lot of attention, in part due to the possible future development of an
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