Efficient secretion of the herpes simplex virus tegument protein VP22 from living mammalian cells

Efficient secretion of the herpes simplex virus tegument protein VP22 from living mammalian cells Many studies show that a tegument protein, VP22, of herpes simplex virus possesses an unusual capacity for intercellular trafficking, while several studies have reported that the intercellular trafficking was observed only in cells after fixation. Therefore, the trafficking ability in living cells remains controversial. To settle the question, we first examined secretion of VP22 in living cells. In this report, we fused VP22 with β-galactosidase (βGal) and investigated the secretion of VP22–βGal in living cells by monitoring βGal activity in the culture medium. Under our conditions, a significant amount of VP22–βGal was detected in the culture medium, and it increased with time. Particularly, 6 days after transfection, 72% of all VP22–βGal expressed was detected in the culture medium. Lactate dehydrogenase assays revealed that leakage of VP22–βGal from damaged cells was not the main cause of the high level of secretion. We thus conclude that VP22 possesses a remarkable ability to be secreted from living cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Efficient secretion of the herpes simplex virus tegument protein VP22 from living mammalian cells

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0094-x
Publisher site
See Article on Publisher Site

Abstract

Many studies show that a tegument protein, VP22, of herpes simplex virus possesses an unusual capacity for intercellular trafficking, while several studies have reported that the intercellular trafficking was observed only in cells after fixation. Therefore, the trafficking ability in living cells remains controversial. To settle the question, we first examined secretion of VP22 in living cells. In this report, we fused VP22 with β-galactosidase (βGal) and investigated the secretion of VP22–βGal in living cells by monitoring βGal activity in the culture medium. Under our conditions, a significant amount of VP22–βGal was detected in the culture medium, and it increased with time. Particularly, 6 days after transfection, 72% of all VP22–βGal expressed was detected in the culture medium. Lactate dehydrogenase assays revealed that leakage of VP22–βGal from damaged cells was not the main cause of the high level of secretion. We thus conclude that VP22 possesses a remarkable ability to be secreted from living cells.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2008

References

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