Efficient plant regeneration in vitro from red leaf beet via organogenesis

Efficient plant regeneration in vitro from red leaf beet via organogenesis An efficient system for in vitro regeneration of red leaf beet, a variety of leaf beet (Beta vulgaris L. var cicla L.) generally used to decorate parterre and to prepare betacyanin, was developed for the first time in the present study. Shoot tip and petiole explants from the sterile seedlings, precultured on Murashige and Skoog (MS) medium with 15 mg/l 6-benzyladenine (BA) and 3% sucrose at 16 °C for 30 days, could form 81.02 and 17.33% translucent nodular (TN) calli, respectively. All TN calli were able to differentiate into adventitious shoots under the same culture conditions. Each explant with TN callus from the shoot tip and petiole could generate 8.65 shoots on average. It was found that both preculture of sterile seedlings and culture of explants at low temperature (16 °C) were vital for TN callus induction and adventitious bud formation of red leaf beet. The best condition for rooting was 0.5-strength MS medium with 10 g/l sucrose. After being transplanted into soil, plantlets grew well and could flower and bear fruits. Histological observation revealed that TN callus was derived from the cells of vascular tissue of the petiole and that adventitious shoots were formed through organogenesis. The factors influencing in vitro micropropagation are also discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Efficient plant regeneration in vitro from red leaf beet via organogenesis

Loading next page...
 
/lp/springer_journal/efficient-plant-regeneration-in-vitro-from-red-leaf-beet-via-FtT4nzAqht
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443709040153
Publisher site
See Article on Publisher Site

Abstract

An efficient system for in vitro regeneration of red leaf beet, a variety of leaf beet (Beta vulgaris L. var cicla L.) generally used to decorate parterre and to prepare betacyanin, was developed for the first time in the present study. Shoot tip and petiole explants from the sterile seedlings, precultured on Murashige and Skoog (MS) medium with 15 mg/l 6-benzyladenine (BA) and 3% sucrose at 16 °C for 30 days, could form 81.02 and 17.33% translucent nodular (TN) calli, respectively. All TN calli were able to differentiate into adventitious shoots under the same culture conditions. Each explant with TN callus from the shoot tip and petiole could generate 8.65 shoots on average. It was found that both preculture of sterile seedlings and culture of explants at low temperature (16 °C) were vital for TN callus induction and adventitious bud formation of red leaf beet. The best condition for rooting was 0.5-strength MS medium with 10 g/l sucrose. After being transplanted into soil, plantlets grew well and could flower and bear fruits. Histological observation revealed that TN callus was derived from the cells of vascular tissue of the petiole and that adventitious shoots were formed through organogenesis. The factors influencing in vitro micropropagation are also discussed.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jun 28, 2009

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off