Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains

Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine... Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10 2 copies of synthetic RNA. Viral RNA from PoRV was detectable at a TCID 50 of 0.01. Significant differences were observed between viral RNA quantification and virus titration results for nine PoRV strains. For nasal and oral swab samples that were collected from experimentally infected pigs, the qRT-PCR assay was more sensitive (87.1–83.9 %) for the detection of positive samples than methods involving isolation of virus. The implementation of highly sensitive assays that yield results quickly will be of great assistance in the eradication of PoRV from Mexico. We also believe that the newly developed qRT-PCR assay will help reduce the spread of this viral infection to other countries. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1672-0
Publisher site
See Article on Publisher Site

Abstract

Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10 2 copies of synthetic RNA. Viral RNA from PoRV was detectable at a TCID 50 of 0.01. Significant differences were observed between viral RNA quantification and virus titration results for nine PoRV strains. For nasal and oral swab samples that were collected from experimentally infected pigs, the qRT-PCR assay was more sensitive (87.1–83.9 %) for the detection of positive samples than methods involving isolation of virus. The implementation of highly sensitive assays that yield results quickly will be of great assistance in the eradication of PoRV from Mexico. We also believe that the newly developed qRT-PCR assay will help reduce the spread of this viral infection to other countries.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2013

References

  • Real time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus
    Boxus, M; Letellier, C; Kerkhofs, P
  • Identification of antigenic variants of the porcine rubulavirus in sera of field swine and their seroprevalence
    Escobar-Lopez, AC; Rivera-Benitez, JF; Castillo-Juarez, H; Ramirez-Mendoza, H; Trujillo-Ortega, ME; Sanchez-Betancourt, JI
  • Multiple factors including subgenomic RNAs and reduced viral protein expression are associated with a persistent infection by porcine rubulavirus (LPMV)
    Hjertner, B; Wiman, AC; Svenda, M; Berg, M; Moreno-Lopez, J; Linne, T

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