Efficacy of Bacillus subtilis native strains for biocontrol of Phytophthora crown and root rot of pistachio in Iran

Efficacy of Bacillus subtilis native strains for biocontrol of Phytophthora crown and root rot of... In Iran, Phytophthora crown and root rot of pistachio trees (also known as gummosis) destroys a significant number of fertile and non- fertile trees each year. To identify potential biocontrol agents effective in controlling pistachio gummosis, 200 soil samples were collected from 19 pistachio growing regions in Kerman province. Out of the 321 strains tested for antagonistic activity against Phytophthora pistaciae, 13 were selected as potential inhibitors of the disease. The tested strains were able to inhibit Phytophthora growth in dual culture, volatile and non-volatile assays by 14–72%, 12–68% and 27–85%, respectively. The highest inhibition was achieved by three strains identified as Bacillus subtilis using phenotypic characteristics, biochemical and physiological tests as well as sequencing the 16S rRNA genomic region for each strain. Co-inoculation experiments of six months old pistachio seedlings with P. pistaciae and the three selected B. subtilis strains reduced mortality rates by up to 80%. B. subtilis strain BSIPR35 was identified as the most promising biocontrol agent in greenhouse experiments. The ability of the selected strains to withstand drought, high temperature and salinity stresses was further tested. The growth of the strains was reduced under abiotic stresses ranging from 22 to 94%. All strains had the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Pathology Springer Journals

Efficacy of Bacillus subtilis native strains for biocontrol of Phytophthora crown and root rot of pistachio in Iran

Loading next page...
 
/lp/springer_journal/efficacy-of-bacillus-subtilis-native-strains-for-biocontrol-of-H32BQBpDeh
Publisher
Springer International Publishing
Copyright
Copyright © 2018 by Sociedade Brasileira de Fitopatologia
Subject
Life Sciences; Plant Pathology
eISSN
1983-2052
D.O.I.
10.1007/s40858-018-0226-0
Publisher site
See Article on Publisher Site

Abstract

In Iran, Phytophthora crown and root rot of pistachio trees (also known as gummosis) destroys a significant number of fertile and non- fertile trees each year. To identify potential biocontrol agents effective in controlling pistachio gummosis, 200 soil samples were collected from 19 pistachio growing regions in Kerman province. Out of the 321 strains tested for antagonistic activity against Phytophthora pistaciae, 13 were selected as potential inhibitors of the disease. The tested strains were able to inhibit Phytophthora growth in dual culture, volatile and non-volatile assays by 14–72%, 12–68% and 27–85%, respectively. The highest inhibition was achieved by three strains identified as Bacillus subtilis using phenotypic characteristics, biochemical and physiological tests as well as sequencing the 16S rRNA genomic region for each strain. Co-inoculation experiments of six months old pistachio seedlings with P. pistaciae and the three selected B. subtilis strains reduced mortality rates by up to 80%. B. subtilis strain BSIPR35 was identified as the most promising biocontrol agent in greenhouse experiments. The ability of the selected strains to withstand drought, high temperature and salinity stresses was further tested. The growth of the strains was reduced under abiotic stresses ranging from 22 to 94%. All strains had the

Journal

Tropical Plant PathologySpringer Journals

Published: Jun 4, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off