Access the full text.
Sign up today, get DeepDyve free for 14 days.
G Neumann, T Watanabe, H Ito, S Watanabe, H Goto, P Gao, M Hughes, DR Perez, R Donis, E Hoffmann, G Hobom, Y Kawaoka (1999)
Generation of influenza A viruses entirely from cloned cDNAsProc Natl Acad Sci USA, 96
E Hoffmann, S Krauss, D Perez, R Webby, RG Webster (2002)
Eight-plasmid system for rapid generation of influenza virus vaccinesVaccine, 20
DE Swayne, ML Perdue, JR Beck, M Garcia, DL Suarez (2000)
Vaccines protect chickens against H5 highly pathogenic avian influenza in the face of genetic changes in field viruses over multiple yearsVet Microbiol, 74
Y Itoh, K Shinya, M Kiso, T Watanabe, Y Sakoda, M Hatta, Y Muramoto, D Tamura, Y Sakai-Tagawa, T Noda, S Sakabe, M Imai, Y Hatta, S Watanabe, C Li, S Yamada, K Fujii, S Murakami, H Imai, S Kakugawa, M Ito, R Takano, K Iwatsuki-Horimoto, M Shimojima, T Horimoto, H Goto, K Takahashi, A Makino, H Ishigaki, M Nakayama, M Okamatsu, D Warshauer, PA Shult, R Saito, H Suzuki, Y Furuta, M Yamashita, K Mitamura, K Nakano, M Nakamura, R Brockman-Schneider, H Mitamura, M Yamazaki, N Sugaya, M Suresh, M Ozawa, G Neumann, J Gern, H Kida, K Ogasawara, Y Kawaoka (2009)
In vitro and in vivo characterization of new swine-origin H1N1 influenza virusesNature, 460
Y Bi, G Fu, J Chen, J Peng, Y Sun, J Wang, J Pu, Y Zhang, H Gao, G Ma, F Tian, IH Brown, J Liu (2010)
Novel swine influenza virus reassortants in pigs, ChinaEmerg Infect Dis, 16
B Manicassamy, RA Medina, R Hai, T Tsibane, S Stertz, E Nistal-Villan, P Palese, CF Basler, A Garcia-Sastre (2010)
Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccinesPLoS Pathog, 6
CS Kyriakis, MR Gramer, F Barbe, J Doorsselaere, K Reeth (2010)
Efficacy of commercial swine influenza vaccines against challenge with a recent European H1N1 field isolateVet Microbiol, 144
H Chen, K Subbarao, D Swayne, Q Chen, X Lu, J Katz, N Cox, Y Matsuoka (2003)
Generation and evaluation of a high-growth reassortant H9N2 influenza A virus as a pandemic vaccine candidateVaccine, 21
RJ Garten, CT Davis, CA Russell, B Shu, S Lindstrom, A Balish, WM Sessions, X Xu, E Skepner, V Deyde, M Okomo-Adhiambo, L Gubareva, J Barnes, CB Smith, SL Emery, MJ Hillman, P Rivailler, J Smagala, M Graaf, DF Burke, RA Fouchier, C Pappas, CM Alpuche-Aranda, H Lopez-Gatell, H Olivera, I Lopez, CA Myers, D Faix, PJ Blair, C Yu, KM Keene, PD Dotson, D Boxrud, AR Sambol, SH Abid, K George, T Bannerman, AL Moore, DJ Stringer, P Blevins, GJ Demmler-Harrison, M Ginsberg, P Kriner, S Waterman, S Smole, HF Guevara, EA Belongia, PA Clark, ST Beatrice, R Donis, J Katz, L Finelli, CB Bridges, M Shaw, DB Jernigan, TM Uyeki, DJ Smith, AI Klimov, NJ Cox (2009)
Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humansScience, 325
H Kida, T Ito, J Yasuda, Y Shimizu, C Itakura, KF Shortridge, Y Kawaoka, RG Webster (1994)
Potential for transmission of avian influenza viruses to pigsJ Gen Virol, 75
LJ Reed, H Muench (1938)
A simple method for estimating fifty percent endpointsAm J Hyg, 27
Y Chen, J Zhang, C Qiao, H Yang, Y Zhang, X Xin, H Chen (2012)
Co-circulation of pandemic 2009 H1N1, classical swine H1N1 and avian-like swine H1N1 influenza viruses in pigs in ChinaInfect Genet Evol, 13
P Kitikoon, D Nilubol, BJ Erickson, BH Janke, TC Hoover, SA Sornsen, EL Thacker (2006)
The immune response and maternal antibody interference to a heterologous H1N1 swine influenza virus infection following vaccinationVet Immunol Immunopathol, 112
AS Lipatov, RJ Webby, EA Govorkova, S Krauss, RG Webster (2005)
Efficacy of H5 influenza vaccines produced by reverse genetics in a lethal mouse modelJ Infect Dis, 191
MH Bikour, E Cornaglia, Y Elazhary (1996)
Evaluation of a protective immunity induced by an inactivated influenza H3N2 vaccine after an intratracheal challenge of pigsCan J Vet Res, 60
W Ma, KM Lager, P Lekcharoensuk, ES Ulery, BH Janke, A Solorzano, RJ Webby, A Garcia-Sastre, JA Richt (2010)
Viral reassortment and transmission after co-infection of pigs with classical H1N1 and triple-reassortant H3N2 swine influenza virusesJ Gen Virol, 91
C Scholtissek (1994)
Source for influenza pandemicsEur J Epidemiol, 10
JJ Skehel, DC Wiley (2000)
Receptor binding and membrane fusion in virus entry: the influenza hemagglutininAnnu Rev Biochem, 69
L Gillim-Ross, K Subbarao (2006)
Emerging respiratory viruses: challenges and vaccine strategiesClin Microbiol Rev, 19
M Xu, Y Huang, J Chen, Z Huang, J Zhang, Y Zhu, S Xie, Q Chen, W Wei, D Yang, X Huang, H Xuan, H Xiang (2011)
Isolation and genetic analysis of a novel triple-reassortant H1N1 influenza virus from a pig in ChinaVet Microbiol, 147
H Yang, Y Chen, J Shi, J Guo, X Xin, J Zhang, D Wang, Y Shu, C Qiao, H Chen (2011)
Reassortant H1N1 influenza virus vaccines protect pigs against pandemic H1N1 influenza virus and H1N2 swine influenza virus challengeVet Microbiol, 152
G Tian, S Zhang, Y Li, Z Bu, P Liu, J Zhou, C Li, J Shi, K Yu, H Chen (2005)
Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse geneticsVirology, 341
RE Shope (1931)
Swine Influenza : III. Filtration experiments and etiologyJ Exp Med, 54
H Yu, RH Hua, Q Zhang, TQ Liu, HL Liu, GX Li, GZ Tong (2008)
Genetic evolution of swine influenza A (H3N2) viruses in China from 1970 to 2006J Clin Microbiol, 46
X Qi, B Pang, CP Lu (2009)
Genetic characterization of H1N1 swine influenza A viruses isolated in eastern ChinaVirus Genes, 39
GJ Smith, D Vijaykrishna, J Bahl, SJ Lycett, M Worobey, OG Pybus, SK Ma, CL Cheung, J Raghwani, S Bhatt, JS Peiris, Y Guan, A Rambaut (2009)
Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemicNature, 459
K Reeth, G Labarque, S Clercq, M Pensaert (2001)
Efficacy of vaccination of pigs with different H1N1 swine influenza viruses using a recent challenge strain and different parameters of protectionVaccine, 19
AL Vincent, KM Lager, BH Janke, MR Gramer, JA Richt (2008)
Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccineVet Microbiol, 126
CW Olsen (2002)
The emergence of novel swine influenza viruses in North AmericaVirus Res, 85
E Fodor, L Devenish, OG Engelhardt, P Palese, GG Brownlee, A Garcia-Sastre (1999)
Rescue of influenza A virus from recombinant DNAJ Virol, 73
Swine influenza (SI) is an acute, highly contagious respiratory disease caused by swine influenza A viruses (SwIVs), and it poses a potential global threat to human health. Classical H1N1 (cH1N1) SwIVs are still circulating and remain the predominant subtype in the swine population in China. In this study, a high-growth reassortant virus (GD/PR8) harboring the hemagglutinin (HA) and neuraminidase (NA) genes from a novel cH1N1 isolate in China, A/Swine/Guangdong/1/2011 (GD/11) and six internal genes from the high-growth A/Puerto Rico/8/34(PR8) virus was generated by plasmid-based reverse genetics and tested as a candidate seed virus for the preparation of an inactivated vaccine. The protective efficacy of this vaccine was evaluated in mice and pigs challenged with GD/11 virus. Prime and boost inoculation of GD/PR8 vaccine yielded high-titer serum hemagglutination inhibiting (HI) antibodies and IgG antibodies for GD/11 in both mice and pigs. Complete protection of mice and pigs against cH1N1 SIV challenge was observed, with significantly fewer lung lesions and reduced viral shedding in vaccine-inoculated animals compared with unvaccinated control animals. Our data demonstrated that the GD/PR8 may serve as the seed virus for a promising SwIVs vaccine to protect the swine population.
Archives of Virology – Springer Journals
Published: Nov 1, 2014
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.