Beet necrotic yellow vein virus (BNYVV) RNA 3 from which all but the 3′ and 5′‘core’ replication origins (promotors) have been deleted replicates when coinoculated to Chenopodium quinoa with viral RNAs 1 and 2. The resulting ‘replicon’ can be used to express inserted heterologous sequences in planta . The effects of alterations of replicon structure on its efficiency of accumulation in planta were examined. Inclusion of up to ∼240 nucleotides of sequence from the region immediately upstream of the core 3′-promoter sequence increased replicon accumulation, suggesting that this region contains specific replication enhancer elements. Insertion of non-viral ‘spacer’ sequences between the core promoters also increased replicon accumulation, provided that no strong secondary structure was present. The highly homologous 3′-terminal core promoters of BNYVV RNAs 1, 2 and 4 could substitute for the RNA 3 core promoter but were generally somewhat less effective. Co-inoculation of full-length RNA 3 but not RNA 4 interfered with accumulation of the RNA 3-based replicons.
Archives of Virology – Springer Journals
Published: Jun 1, 1999
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