Effects of structural modifications upon the accumulation in planta of replicons derived from beet necrotic yellow vein virus RNA 3

Effects of structural modifications upon the accumulation in planta of replicons derived from... Beet necrotic yellow vein virus (BNYVV) RNA 3 from which all but the 3′ and 5′‘core’ replication origins (promotors) have been deleted replicates when coinoculated to Chenopodium quinoa with viral RNAs 1 and 2. The resulting ‘replicon’ can be used to express inserted heterologous sequences in planta . The effects of alterations of replicon structure on its efficiency of accumulation in planta were examined. Inclusion of up to ∼240 nucleotides of sequence from the region immediately upstream of the core 3′-promoter sequence increased replicon accumulation, suggesting that this region contains specific replication enhancer elements. Insertion of non-viral ‘spacer’ sequences between the core promoters also increased replicon accumulation, provided that no strong secondary structure was present. The highly homologous 3′-terminal core promoters of BNYVV RNAs 1, 2 and 4 could substitute for the RNA 3 core promoter but were generally somewhat less effective. Co-inoculation of full-length RNA 3 but not RNA 4 interfered with accumulation of the RNA 3-based replicons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Effects of structural modifications upon the accumulation in planta of replicons derived from beet necrotic yellow vein virus RNA 3

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1999 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050579
Publisher site
See Article on Publisher Site

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