Effects of hepatitis B virus X gene on apoptosis and expression of immune molecules of human proximal tubular epithelial cells

Effects of hepatitis B virus X gene on apoptosis and expression of immune molecules of human... The hepatitis B virus X protein (HBx) is the most important determinant in viral pathogenesis. HBx regulates HBV replication, cellular transcription, signal transduction, proteasome activity and cell cycle progression. In this study, HK-2 cells were transiently transfected with the HBx gene using a eukaryotic vector, pcDNA3.1/myc-HBx. Transfection with the HBx gene increased the number of apoptotic cells. In addition, cultured HK-2 cells became irregular in shape. The expression of α-SMA and E-cadherin, TLR4, MHC-II and CD40 was increased. The transfection resulted in increased IL-1, IL-6, TNF-α and IFN-γ levels in the supernatant and decreased IL-4 in the supernatant. In conclusion, overexpression of the HBx gene in renal tubular epithelial cells induces apoptosis of HK-2 cells and promotes epithelial-mesenchymal transdifferentiation. HBx transfection upregulates the expression of immune molecules in renal tubular epithelial cells and induces an imbalance in cytokine levels. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Effects of hepatitis B virus X gene on apoptosis and expression of immune molecules of human proximal tubular epithelial cells

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1759-7
Publisher site
See Article on Publisher Site

Abstract

The hepatitis B virus X protein (HBx) is the most important determinant in viral pathogenesis. HBx regulates HBV replication, cellular transcription, signal transduction, proteasome activity and cell cycle progression. In this study, HK-2 cells were transiently transfected with the HBx gene using a eukaryotic vector, pcDNA3.1/myc-HBx. Transfection with the HBx gene increased the number of apoptotic cells. In addition, cultured HK-2 cells became irregular in shape. The expression of α-SMA and E-cadherin, TLR4, MHC-II and CD40 was increased. The transfection resulted in increased IL-1, IL-6, TNF-α and IFN-γ levels in the supernatant and decreased IL-4 in the supernatant. In conclusion, overexpression of the HBx gene in renal tubular epithelial cells induces apoptosis of HK-2 cells and promotes epithelial-mesenchymal transdifferentiation. HBx transfection upregulates the expression of immune molecules in renal tubular epithelial cells and induces an imbalance in cytokine levels.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2013

References

  • Hepatitis B virus X protein: a multifunctional viral regulator
    Murakami, S
  • Hepatitis B virus X protein blunts senescence-like growth arrest of human hepatocellular carcinoma by reducing Notch1 cleavage
    Xu, J; Yun, X; Jiang, J; Wei, Y; Wu, Y; Zhang, W; Liu, Y; Wang, W; Wen, Y; Gu, J
  • Selective transcriptional regulations in the human liver cell by hepatitis B viral X protein
    Han, J; Yoo, HY; Choi, BH; Roh, HM
  • The role of the toll-like receptor TLR4 in hepatitis B virus-associated glomerulonephritis
    Zhou, Y; Zhu, N; Wang, X; Wang, L; Gu, LJ; Yuan, WJ

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