Effects of He-Ne laser on nuclear F-actin under UV-B radiation

Effects of He-Ne laser on nuclear F-actin under UV-B radiation The alleviating effects of He-Ne laser on damage of nuclear actin filaments organization caused by ultraviolet-B (UV-B) light were investigated. Wheat (Triticum aestivum L. cv. ML7113) seedlings were irradiated with enhanced UV-B (10.08 kJ/(m2 d)), or He-Ne laser (5.43 mW/mm2), or combination of He-Ne laser and UV-B. The existence, distribution and relative content of F-actin in the nucleus were demonstrated by confocal microscopy and flow cytometry after fluorescence labeling with FITC-phalloidin. It was shown that the enhanced UV-B radiation significantly increased the diffuse fluorescence staining that resulted in bright fragments of F-actin being observed in the nucleoplasm. The damage caused by UV-B radiation was partially alleviated when the He-Ne laser was used: it could be seen that F-actin distribution in experiment was similar to that in the control. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Effects of He-Ne laser on nuclear F-actin under UV-B radiation

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Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715060047
Publisher site
See Article on Publisher Site

Abstract

The alleviating effects of He-Ne laser on damage of nuclear actin filaments organization caused by ultraviolet-B (UV-B) light were investigated. Wheat (Triticum aestivum L. cv. ML7113) seedlings were irradiated with enhanced UV-B (10.08 kJ/(m2 d)), or He-Ne laser (5.43 mW/mm2), or combination of He-Ne laser and UV-B. The existence, distribution and relative content of F-actin in the nucleus were demonstrated by confocal microscopy and flow cytometry after fluorescence labeling with FITC-phalloidin. It was shown that the enhanced UV-B radiation significantly increased the diffuse fluorescence staining that resulted in bright fragments of F-actin being observed in the nucleoplasm. The damage caused by UV-B radiation was partially alleviated when the He-Ne laser was used: it could be seen that F-actin distribution in experiment was similar to that in the control.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 18, 2015

References

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