Effects of free amino acids on the photosynthetic carbon metabolism of symbiotic dinoflagellates

Effects of free amino acids on the photosynthetic carbon metabolism of symbiotic dinoflagellates Synthetic host factor (SHF) was used in parallel with crude cnidarian’s host factor (CHF) to investigate their effects on both photosynthetic carbon metabolism and the first step of lipid synthesis in the symbiotic dinoflagellates Symbiodium purchrorum of the sea anemone Aiptasia pulchella. Several species have been studied, namely, sea anemone A. pulchella, reef coral Pocillopora damicornis, and green alga Chlamydomonas reinhardii. Both short-term and long-term experiments with radioactive carbon have shown a higher rate of the alga 14C photoaccumulation with host factor(s) than of dinoflagellates located in artificial sea water (ASW) alone. In dinoflagellates incubated with both ASW and CHF, 14C-labeled glycerol was detectable after 15 s of alga illumination. In dinoflagellates isolated from P. damicornis and incubated in CHF and SHF and in dinoflagellates isolated from A. pulchella and incubated in CHF, a higher percentage of 14C was found in the glycerol as compared to the ASW trial. At the same time, in ASW trial the radioactive label was primarily located in ethanol-soluble lipid fraction. Similar results were observed when dinoflagellates isolated from P. damicornis were incubated with aspartate or glutamate. But there was no effect with taurine, serine, valine, glycine, or lysine. C. reinhardii, incubated in salt-free CHF, partitioned a greater percent of 14C into the glycerol and less into the ethanol-soluble lipids as compared to the corresponding control incubations. The amount of 14C in neutral and polar lipids was identical in that in A. pulchella dinoflagellates incubated in ASW or CHF. The arrays of neutral 14C-lipids produced under both ASW alone and ASW with CHF conditions, and over time were not significantly distinguishable. Host factors appeared to provide an optimum environment to sustain maximum metabolic efficiency. A biochemical model, based on the quantitative and qualitative assessment of carbon pathways in dinoflagellates incubated both in host factor and sea water alone, is presented. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Effects of free amino acids on the photosynthetic carbon metabolism of symbiotic dinoflagellates

Loading next page...
 
/lp/springer_journal/effects-of-free-amino-acids-on-the-photosynthetic-carbon-metabolism-of-W2cKSzpYY7
Publisher
Nauka/Interperiodica
Copyright
Copyright © 2007 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443707020033
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial