Effects of Dipole Potential Modifiers on Heterogenic Lipid Bilayers

Effects of Dipole Potential Modifiers on Heterogenic Lipid Bilayers In this work, we examine the ability of dipole modifiers, flavonoids, and RH dyes to affect the dipole potential (φ d) and phase separation in membranes composed of ternary mixtures of POPC with different sphingolipids and sterols. Changes in the steady-state conductance induced by cation–ionophore complexes have been measured to evaluate the changes in dipole potential of planar lipid bilayers. Confocal fluorescence microscopy has been employed to investigate lipid segregation in giant unilamellar vesicles. The effects of flavonoids on φ d depend on lipid composition and dipole modifier type. The effectiveness of RH dyes to increase φ d depends on sphingolipid type but is not influenced by sterol content. Tested modifiers lead to partial or complete disruption of gel domains in bilayers composed of POPC, sphingomyelin, and cholesterol. Substitution of cholesterol to ergosterol or 7-dehydrocholesterol leads to a loss of fluidizing effects of modifiers except phloretin. This may be due to various compositions of gel domains. The lack of influence of modifiers on phase scenario in vesicles composed of ternary mixtures of POPC, cholesterol, and phytosphingosine or sphinganine is related to an absence of gel-like phase. It was concluded that the membrane lateral heterogeneity affects the dipole-modifying abilities of the agents that influence the magnitude of φ d by intercalation into the bilayer and orientation of its own large dipole moments (phloretin and RH dyes). The efficacy of modifiers that do not penetrate deeply and affect φ d through water adsorption (phlorizin, quercetin, and myricetin) is not influenced by lateral heterogeneity of membrane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Effects of Dipole Potential Modifiers on Heterogenic Lipid Bilayers

Loading next page...
 
/lp/springer_journal/effects-of-dipole-potential-modifiers-on-heterogenic-lipid-bilayers-cTRAX08EZp
Publisher
Springer US
Copyright
Copyright © 2015 by Springer Science+Business Media New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-015-9852-3
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial