ISSN 10214437, Russian Journal of Plant Physiology, 2012, Vol. 59, No. 1, pp. 79–87. © Pleiades Publishing, Ltd., 2012.
Original Russian Text © N.S. Zakharchenko, S.V. Pigoleva, V.V. Kochetkov, M.A. Chepurnova, O.V. D’yachenko, A.A. Lebedeva, A.V. Zakharchenko, I.F. Puntus, A.M. Boronin,
Ya.I. Bur’yanov, 2012, published in Fiziologiya Rastenii, 2012, Vol. 59, No. 1, pp. 89–98.
An increase in plant tolerance to various stressors is
one of the important tasks of modern plant physiology
and the basis for improving efficiency of agriculture.
In addition to direct breeding and application of
chemicals, plant protection against phytopathogens
can be achieved by using genetic engineering technol
ogies  and microorganisms . For the biological
safety purposes, improved technologies of microor
ganism application seems to be really promising.
Under natural conditions, plants live in close associa
tion with the complex of soil microorganisms, which
have a stimulating effect on plant growth and develop
ment [3, 4]. This is related to the capability of micro
organisms of nitrogen fixation, production of physio
logically active compounds, mobilizing nutrients from
the soil, suppression of phytopathogen growth, and
detoxification of xenobiotics. The list of microorgan
isms beneficial for plant growth and their tolerance to
biotic and abiotic stressors is small, and the expanding
of this list is a promising research task.
The objective of our work was to investigate the
influence of plant colonization with associative
pseudomonads and methylobacteria on their growth
and resistance to some biotic and abiotic stressors.
MATERIALS AND METHODS
Experiments were performed on
Mill., cv. Dzhin),
L., cv. Slava
1305), rape (
L., cv. Galant), the com
mon ice plant (
and tobacco (
L., cv. Samsun).
Seeds were sterilized in 70% ethanol for 1.5 min,
then in 20% sodium hypochlorite for 2 min; thereafter,
seeds were washed three times (10 min each) with ster
ile distilled water and germinated on hormonefree
Murashige and Skoog (MS) medium  in darkness
during first 5 days and later in the light and a 16h pho
. In 3 weeks, apical segments 5–7 mm
in size were cut of sterile plants and transferred for
Effects of Associative Pseudomonads and Methylobacteria
on Plant Growth and Resistance to Phytopathogens
N. S. Zakharchenko
, S. V. Pigoleva
, V. V. Kochetkov
, M. A. Chepurnova
, O. V. D’yachenko
A. A. Lebedeva
, A. V. Zakharchenko
, I. F. Puntus
, A. M. Boronin
, and Ya. I. Bur’yanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch), Russian Academy of Sciences,
pr. Nauki 6, Pushchino, Moscow oblast, 142290 Russia;
Skryabin Institute of the Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences,
Pushchino, Moscow oblast, Russia
Tula State University, Tula, Russia
Pushchino State University, Pushchino, Moscow oblast, Russia
Received February 27, 2011
—The in vivo and in vitro interactions between tobacco (
L.), tomato (
Mill.), cabbage (
L.), rape (
L.), and the common ice
L.) and bacteria
Pseudomonas aureofaciens, P. putida
were studied. Stable associations of these microorganisms with plants are demonstrated. Colo
nized plants were characterized by accelerated growth, more efficient rooting, better adaptation to in vivo
conditions, and enhanced resistance to bacterial and fungal phytopathogens (
Erwinia carotovora, Sclerotinia
). Plants colonized by bacteria resistant to kanamycin and naphtha
lene can grow steadily on the medium containing these compounds. The results obtained indicate a promising
usage of beneficial associative microorganisms for the development of technologies for plant protection
against biotic and abiotic stressors.
: higher plants, associative microorganisms, phytopathogens, colonization.
: BA—benzyladenine; CFU—colonyforming unit;
Km—kanamycin; MS—Murashige and Skoog nutrient medium.