ISSN 10214437, Russian Journal of Plant Physiology, 2011, Vol. 58, No. 6, pp. 1069–1073. © Pleiades Publishing, Ltd., 2011.
Original Russian Text © E.O. Polovinkina, E.A. Kal’yasova, Yu.V. Sinitsina, A.P. Veselov, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 6, pp. 930–934.
Weak lowfrequency magnetic field is a penetrating
environmental agent, which simultaneously affect
both systemic mechanisms and structural and meta
bolic processes on the levels of cells and organisms .
Specific cellular sensors percepting external signals
about changes in the magnetic field characteristics and
transforming them in the living cell responses on the
organismal level are not elucidated with rare excep
tions. However, such fields are widely used in magne
totherapy, often without understanding the mecha
nisms of their action.
Currently, changes of biomembrane properties are
widely discussed as the main effect of magnetic fields
on the living cell, in particular, on the proton pump
functioning  amd membrane lipid composition .
Effects of magnetic fields (MFs) could be related to
uncoupling of free radical processes in membranes
and enhanced ROS generation [4, 5]. MFs could also
affect metalloenzymes. It has been experimentally
proven that MF could change activities of some
enzymes: catalase, superoxide dismutase (SOD), glu
tathione reductase, glutathione transferase . It was
also reported that cell metabolic activity could be
changed because of resonance phenomena at the
action of combined MFs on metabolically importants
ions: calcium [7–10], potassium , and others.
The investigation of cell and cellular structure
parameters, including physical and chemical state of
membranes, is a priority task in the studies of fine
interaction between MF of various intensity and living
organisms. Physical signals could be percepted by the
system of peroxidation homeostasis maintaining the
balance between pro and antioxidants in the cell. The
state of this homeostasis is assessed by the content of
intermediate and final products of lipid peroxidation
(dienoic conjugates (DC), MDA, Schiff bases), by
activities of antioxidant enzymes (for example, super
oxide dismutase (SOD), glutathione transferase, per
oxidases), and by the content of lowmolecular anti
oxidants (ascorbic acid (Asc), tocopherols, glu
tathione, etc.) [12, 13].
The objective of this work is the assessment of
changes in the chloroplast peroxide homeostasis under
the influence of weal MF of various intensities used in
MATERIALS AND METHODS
Experiments were performed with twoweekold
L., cvs. Albumen and Shustrik)
plants grown under laboratory conditions (an illumi
nance of 16 klx, a 16h photoperiod, room tempera
Effect of Weak Pulse Magnetic Fields on Lipid Peroxidation
and Activities of Antioxidant Complex Components
in Pea Chloroplasts
E. O. Polovinkina, E. A. Kal’yasova, Yu. V. Sinitsina, and A. P. Veselov
Faculty of Biology, Lobachevsky State University of Nizhnii Novgorod, pr. Gagarina 23, Nizhnii Novgorod, 603950 Russia;
Received February 27, 2011
—The effects of magnetic fields (MF) with different intensity, which are applied in magnetotherapy,
on the redox homeostasis in chloroplasts of twoweekold pea (
L.) cvs. Albumen and Shustrik
were studied. The seedlings were treated with weak MF produced by the generators UMTIZF or that from
ElectroBiology firm for 15, 30, 60, and 120 min. Production of early and final products of lipid peroxidation
(POL) (dienoic conjugates, MDA, and Schiff bases) and also activity of superoxide dismutase (SOD) and the
content of ascorbic acid (Asc) in chloroplasts were assessed. The stronger pulse MF (PMF1) induced a stable
decrease of POL products, whereas the weaker PMF2 induced reversible accumulation of dienoic conju
gates and Schiff bases. PMF1 successively activated SOD, inhibited it, and finally stabilized its activity at the
control level. Similar treatment with PMF2 induced similar SOD activity changes but did not inhibit SOD.
The faster response of Asc to PMF1 was noted.
Keywords: Pisum sativum
, weak magnetic fields, chloroplasts, lipid peroxidation, antioxidant defense, super
oxide dismutase, ascorbic acid.
: Asc—ascorbic acid; DC—dienic conjugates;
MF—magnetic field; PMF1 and PMF2—relatively stronger
and weaker pulse field, respectively; SchB—Schiff base; SOD—