ISSN 10214437, Russian Journal of Plant Physiology, 2011, Vol. 58, No. 4, pp. 603–614. © Pleiades Publishing, Ltd., 2011.
Flavonoids and resveratrol are important second
ary metabolites in plants. Although flavonoids are
ubiquitous in the plant kingdom, resveratrol is specific
to only certain plant families, such as Pinaceae, Legu
minosae, and Vitaceae [1–3]. In grapevines, resvera
trol accumulates in leaves and berries of plants sub
jected to biotic stresses (fungal infection by
cinerea, Plasmopara viticola
abiotic stresses (UVC irradiation; chemical treat
ments, such as treatments with aluminum chloride or
jasmonic acid; wounding; and ozone exposure) [4–7].
Moreover, resveratrol and its glycoside are considered
to have beneficial effects on human health because
they possess antiinflammatory, antiplatelet, and anti
carcinogenic activities .
Resveratrol and flavonoid syntheses have a com
mon origin since both derive in the general phenylpro
This text was submitted by the authors in English.
These authors contributed equally to this work.
panoid metabolism. Resveratrol is synthesized by stil
bene synthase (STS, EC 18.104.22.168), which condenses
one molecule of coumaroylCoA and three molecules
of malonylCoA , while flavonoid formation occurs
due to the action of chalcone synthase (CHS,
EC 22.214.171.124) on coumaroylCoA and three molecules
of malonylCoA via cleavage of three carbon dioxide
molecules. Resveratrol has antifungal activity, and its
synthesis could be induced in response to pathogen
infections; thus, it is a member of the natural phytoal
exin group. For this reason, resveratrol has attracted
attention as a plant defense agent against fungal infec
tions. Transgenic monocot and dicot plants producing
resveratrol by overexpression of
genes showed an
increased resistance against various fungal infections [10,
gene belongs to a small multigene family,
several members of which have been cloned .
In recent years, STS has been the focus of studies
on the cloning of gene and/or cDNA and the regula
tion at the transcriptional level . In some cases,
transformed plants expressing a grape
to be more resistant to their pathogens [10, 11]. More
recently, the localization of STS in
grape berry development was addressed with an anti
body specifically developed against grape STS1
enzyme, and its predominant distributing in the cell
wall within exocarp tissues was demonstrated [14, 15].
Effect of UVC Irradiation on Stilbene Synthase Localization
in Young Grape Plants
, F. Fang
, H. R. Yang
, and W. D. Huang
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
fax: +8601062737553; email: email@example.com
Institute of AgroFood Science and Technology, Chinese Academy of Agricultural Sciences,
Yuan Ming Yuan West Road 2, Haidian District, Beijing 100193, China
Received April 2, 2010
—Stilbene synthase (STS) is a pivotal enzyme that catalyzes the biosynthesis of resveratrol and could
be induced by UVC irradiation. However, at present the effect of UVC irradiation on tissue and subcellular
localization of STS is not studied. In this work, subcellular localization of STS in young grape (
cv. Cabernet Sauvignon) plants exposed to UVC was examined immunohistochemically using a polyclonal
antibody raised against grape berry STS. The immunohistochemical analysis showed that the UVinduced
STS occurred in palisade tissues of grape leaves and phloem tissues of grape leaf veins, stems, and roots. At
the subcellular level, the enhanced STS stimulated by UVC light was visualized in the cell walls, chloroplasts
(plastids), cytoplasm, and nucleus of the phloem (stems and roots), while only in chloroplasts in mesophyll
cells. This distribution pattern of STS arising in response to UVC irradiation may be closely involved in its
defense function, which needs much more indepth evidence to confirm.
, immunohistochemical localization, stilbene synthase, subcellular localization, UVC.
: Chl—chloroplast; Co—cortex; CW—cell wall;
Cyt—cytoplasm; EP—epidermis; En—endodermis; LE—lower
epidermis; Nu—nucleolus; P—plastid; PiR—pith ray; PPh—
primary phloem; PT—palisade tissue; PX—protoxylem; S—
starch grain; Sc—sclerenchyma; ST—spongy tissue; SX—sec
ondary xylem; UE—upper epidermis; V—vacuole; VB—vascu
lar bundle; VC—vascular cylinder; X—xylem.