Effect of the ABRUPTUS/PINOID gene on expression of the LEAFY gene in Arabidopsis thaliana

Effect of the ABRUPTUS/PINOID gene on expression of the LEAFY gene in Arabidopsis thaliana The nucleotide sequence was analyzed for the temperature-sensitive allele abruptus (abr), which distorts polar auxin transport (PAT) in the inflorescence. The mutation C → T was found in the second exon and led to an amino acid substitution (glycin → glutamic acid) in the conserved domain of protein kinase encoded by the ABRUPTUS/PINOID (ABR/PID) gene. QRT-PCR revealed a 100-fold decrease in transcription of the LEAFY (LFY) gene in the abr mutant with high expressivity of the mutant character; transcription of the fused LFY::GUS gene was also low in the mutant. The results agree with data of the phenotypic analysis of the abr lfy double mutant and testify to an important role of auxin gradients in regulating the expression pattern of the LFY gene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Effect of the ABRUPTUS/PINOID gene on expression of the LEAFY gene in Arabidopsis thaliana

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2005 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1007/s11177-005-0108-7
Publisher site
See Article on Publisher Site

Abstract

The nucleotide sequence was analyzed for the temperature-sensitive allele abruptus (abr), which distorts polar auxin transport (PAT) in the inflorescence. The mutation C → T was found in the second exon and led to an amino acid substitution (glycin → glutamic acid) in the conserved domain of protein kinase encoded by the ABRUPTUS/PINOID (ABR/PID) gene. QRT-PCR revealed a 100-fold decrease in transcription of the LEAFY (LFY) gene in the abr mutant with high expressivity of the mutant character; transcription of the fused LFY::GUS gene was also low in the mutant. The results agree with data of the phenotypic analysis of the abr lfy double mutant and testify to an important role of auxin gradients in regulating the expression pattern of the LFY gene.

Journal

Russian Journal of GeneticsSpringer Journals

Published: May 10, 2005

References

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